Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells.

Deducing the electron-beam diameter in a laser-plasma accelerator using x-ray betatron radiation.

We investigate the properties of a laser-plasma electron accelerator as a bright source of keV x-ray radiation. During the interaction, the electrons undergo betatron oscillations and from the carefully measured x-ray spectrum the oscillation amplitude of the electrons can be deduced which decreases with increasing electron energies. From the oscillation amplitude and the independently measured x-ray source size of (1.

Nanosecond dynamics of photoexcited lyotropic liquid crystal structures.

Home-based soft X-ray time-resolved diffraction (TR-SXD) experiments with nanosecond time resolution (10 ns) and nanometer spatial resolution were carried out at a tabletop soft X-ray plasma source (2.7-5.9 nm).

Time-resolved soft X-ray diffraction reveals transient structural distortions of ternary liquid crystals.

Home-based soft X-ray time-resolved scattering experiments with nanosecond time resolution (10 ns) and nanometer spatial resolution were carried out at a table top soft X-ray plasma source (2.2-5.2 nm).

Radiation damage resistance of AlGaN detectors for applications in the extreme-ultraviolet spectral range.

We report on the fabrication of aluminum gallium nitride (AlGaN) Schottky-photodiode-based detectors. AlGaN layers were grown using metal-organic chemical vapor deposition (MOCVD) on Si(111) wafers. The diodes were characterized at a wavelength of 13.

Solid supported multicomponent lipid membranes studied by x-ray spectromicroscopy.

This article addresses the lateral organization of two-component lipid membranes deposited on a solid support with the addition of colloidal particles. The authors have applied synchrotron-based scanning transmission soft x-ray spectromicroscopy to image thin lipid layer patches with bound microspheres coated by a charged monolayer. The ability and current limits of scanning transmission x-ray spectromicroscopy to examine samples under physiologically relevant conditions in the presence of excess water have been tested.

XUV laser-plasma source based on solid Ar filament.

We present a laser driven soft x-ray source based on a novel solid argon filament. The continuously flowing micron-sized filament (diameter approximately 56 microm, flow speed approximately 5 mms) was used as a laser target in order to generate a plasma source of high brightness in the "water window" (2.2-4.