Patient-Experienced Quality During Postoperative Pain Management - A Phenomenological-Hermeneutic Study.

Purpose: The aim of this study was to describe an in-depth understanding of patients' experiences and perspectives after use of the erector spinae plane block (ESP) in patients undergoing laparoscopic hemicolectomy due to malignant disease. Knowledge gained throughout the study aimed to ensure increased patient-experienced quality, patient safety and adequate post-surgery pain treatment.

Design: A qualitative approach based on a phenomenological-hermeneutic framework inspired by Paul Ricoeur's perspectives was used.

The effects of different potato dextrose agar media on secondary metabolite production in Fusarium.

Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium. Commercially, PDA is available from multiple retailers offering virtually the same product. These media, however, could contain small differences in composition of nutrients affecting the expression of secondary metabolites.

Is a computer-based measurement method superior to a recommended manual method by the ROHO(®) Group to assess pressure in the sitting position?

Background: In clinical practice, a manual-based assessment is standard procedure to adjust the air pressure in the ROHO(®) cushion in seated position. Recently, a computerized pressure system method was developed to support the clinical adjustment of air in the ROHO(®) cushion to reduce the pressure in the seated position. To our knowledge, no studies have investigated the reliability between the manual method mostly used in clinical practice and the alternative computer-based assessment of the pressure in the seated position.

Elliptical posts allow for detailed control of non-equibiaxial straining of cell cultures.

Background: A modification of the Flexcell system that allows imposition of homogenous, controlled non-equibiaxial strains to cell cultures is developed and experimentally validated. The Flexcell system by default applies equibiaxial strain to cell cultures, meaning no shear strain, while soft tissue cells in vivo are subjected to a range of mechanical deformations including shear strain caused by activities of daily living. Shear strains are suspected to play an important role in tissue necrosis.

Uniaxial cyclic strain drives assembly and differentiation of skeletal myocytes.

Ex vivo engineering of skeletal muscle represents an exciting new area of biotechnology. Although the ability of skeletal muscle cells to sense and respond to mechanical forces is well known, strategies based on the use of mechanical stimuli to optimize myogenesis in vitro remain limited. In this work, we describe a simple but powerful method based on uniaxial cyclic tensile strain (CTS) to induce assembly and differentiation of skeletal myocytes in vitro.

The variability and complexity of sitting postural control are associated with discomfort.

The present investigation examined the variability of sitting postural movement in relation to the development of perceived discomfort by means of linear and nonlinear analysis. Nine male subjects participated in this study. Discomfort ratings, kinetic and kinematics data were recorded during prolonged sitting.

Missing links in pressure ulcer research--an interdisciplinary overview.

This paper surveys the literature on the etiology of sitting-acquired deep tissue pressure ulcers from three different viewpoints. The first viewpoint is identification of risk factors related to seated posture. The second viewpoint focuses on the external factors that can cause necrosis to human cells, such as ischemia and compression.

Regional differences in expression of specific markers for human embryonic stem cells.

Characterization of human embryonic stem cell (hESC) lines derived from the inner cell masses of blastocysts generally includes expression analysis of markers such as OCT4, NANOG, SSEA3, SSEA4, TRA-1-60 and TRA-1-81. Expression is usually detected by immunocytochemical staining of entire colonies of hESC, using one colony for each individual marker. Four newly established hESC lines showed the expected expression pattern and were capable of differentiating into the three germ layers in vitro.

Tesmin transcription is regulated differently during male and female meiosis.

Tesmin is a protein with homology to the metal-binding motif of the metallothionein protein family. Tesmin has been described as a testis-specific transcript, which starts to accumulate in 8-day-old mouse spermatocytes. Herein, a differential display comparing meiotic gene expression in embryonic ovaries and mature testes also revealed the presence of the Tesmin transcript in fetal ovaries as well as in fetal and adult heart.

Mutational analysis of the human FATE gene in 144 infertile men.

The FATE gene maps to Xq28 where one case of a translocation breakpoint has been found in an infertile man. Moreover, the FATE promoter contains a putative SF-1-binding site, and FATE has been proposed as representing a target gene of SF-1 in testicular development or germ cell differentiation. This study presents a complete mutational screening of the FATE gene in a random group of 144 infertile males.