Publications by authors named "Christian Kazenwadel"
Article Synopsis
- Phenoxy radical coupling reactions, guided by dirigent proteins, are essential for lignan biosynthesis, creating specific compounds like (+) or (-)-pinoresinol from coniferyl alcohol.
- A new high-yield production process using Pichia pastoris has significantly increased the amount of the dirigent protein AtDIR6 to over 47 mg L⁻¹, improving upon previous methods by more than 250 times.
- Biochemical analysis confirmed that AtDIR6 produced in P. pastoris closely matches that from plant cultures in terms of structure and function, highlighting the importance of glycan structures for the protein's activity and stability.
Immobilized cutinase HiC from the ascomycete Humicola insolens was applied as a novel biocatalyst for the synthesis of functionalized acryclic esters by transesterification. As a model reaction, transesterification of methyl acrylate with 6-mercapto-1-hexanol at a high molar ratio in a solvent free system was chosen. Besides two minor Michael-addition by-products, 6-mercaptohexyl acrylic ester was identified as the main product with the thiol as the functional end group.
View Article and Find Full Text PDFThe cutinase CUTAB1 was cloned from a cutin induced culture of Alternaria brassicicola and heterologously expressed in Pichia pastoris under the control of the methanol-inducible AOX1 promoter. From a 400-ml culture, 36 mg of purified recombinant enzyme were obtained. Biochemical characterization revealed highest catalytic activity of the enzyme at 40 degrees C and pH 7-9 using p-nitrophenyl palmitate (p-NPP) as substrate.
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