Publications by authors named "Christian Grad"
Identification of Genetic Predispositions Related to Ionizing Radiation in Primary Human Skin Fibroblasts From Survivors of Childhood and Second Primary Cancer as Well as Cancer-Free Controls: Protocol for the Nested Case-Control Study KiKme.
JMIR Res Protoc
November 2021
Background: Therapy for a first primary neoplasm (FPN) in childhood with high doses of ionizing radiation is an established risk factor for second primary neoplasms (SPN). An association between exposure to low doses and childhood cancer is also suggested; however, results are inconsistent. As only subgroups of children with FPNs develop SPNs, an interaction between radiation, genetic, and other risk factors is presumed to influence cancer development.
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- The study investigates how human fibroblasts respond to ionizing radiation by analyzing gene expression changes at two time points post-exposure (2 hours and 4 hours) after being irradiated with different doses of X-rays (2Gy and 0.05Gy).
- It uses RNA sequencing to identify differentially expressed genes and perform pathway analyses to understand the biological effects and mechanisms behind radiation exposure.
- Findings show that more genes were differentially expressed at the 4-hour mark, especially with high-dose radiation, affecting pathways related to cancer signaling and metabolism, while low-dose exposure didn’t produce significant changes at either time point.
Current in vitro assays typically poorly predict cardiac liability as they focus on single ion channels overexpressed in cell lines. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), on the other hand, provide a unique opportunity for drug testing on human cardiomyocytes using high-throughput systems. However, these cells can differ from adult cardiomyocytes in their ion channel expression and, therefore, electrophysiologic properties.
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