Publications by authors named "Christian C Kramme"
Donor side effects experienced under minimal controlled ovarian stimulation with in vitro maturation vs. conventional controlled ovarian stimulation for in vitro fertilization treatment.
F S Sci
August 2024
Objective: To evaluate oocyte retrieval experiences and side effects under minimally controlled ovarian stimulation (COS) treatment for in vitro maturation (IVM) of oocytes compared with conventional COS treatment.
Design: A retrospective survey study.
Setting: Clinical in vitro fertilization treatment center.
Objective: To demonstrate clinical techniques for in vitro maturation (IVM) treatment, including stimulation recommendations, small follicle pick-up procedures, and compact cumulus-oocyte complex (COC) search practice.
Design: This video utilizes live-action footage from surgery and embryology practice for a representative IVM treatment cycle, with step-by-step instructions and recommendations for practice procedures.
Setting: In vitro fertilization (IVF) clinic.
Purpose: Determine if the gene expression profiles of ovarian support cells (OSCs) and cumulus-free oocytes are bidirectionally influenced by co-culture during in vitro maturation (IVM).
Methods: Fertility patients aged 25 to 45 years old undergoing conventional ovarian stimulation donated denuded immature oocytes for research. Oocytes were randomly allocated to either OSC-IVM culture (intervention) or Media-IVM culture (control) for 24-28 h.
Article Synopsis
- The study investigates whether co-culturing human oocytes with ovarian support cells (OSCs) from human-induced pluripotent stem cells (hiPSCs) enhances the maturation and developmental potential of the oocytes compared to a standard in vitro maturation (IVM) system.
- Results indicate that oocytes matured using OSC-IVM show significantly higher rates of metaphase II (MII) formation and successful blastocyst development, outperforming traditional IVM methods.
- The research included 67 donors, focusing on women aged 19 to 37, and was conducted over 15 months to evaluate various fertility parameters and compare outcomes between OSC-IVM and control conditions.
An in vitro model of human ovarian follicles would greatly benefit the study of female reproduction. Ovarian development requires the combination of germ cells and several types of somatic cells. Among these, granulosa cells play a key role in follicle formation and support for oogenesis.
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