Isotachophoretic free-flow electrophoretic focusing and SERS detection of myoglobin inside a miniaturized device.

A new approach for label-free optical sample detection via surface enhanced Raman spectroscopy (SERS) inside a free-flow electrophoresis (FFE) chip is presented in this work.

Force microscopy analysis using chemometric tools.

In this paper we report the first application of multivariate data analysis techniques to force spectrometry measurement sets to enable the physicochemical assignment of spatially ordered multi-component systems. Principal component analysis (PCA) and hierarchical clustering techniques were used to reveal hidden chemical information within force-distance curves generated by high spatial resolution force microscopy. Two experimental samples were analyzed: (i) a two-component system of cytochrome c proteins on a mica surface, and (ii) a three-component system of avidin protein islands positioned on a gold and glass surface.