Development of 3D gingival in vitro models using primary gingival cells.

Since March 2013, animal testing for toxicity evaluation of cosmetic ingredients is banned in Europe. This directive applies to all personal care ingredients including oral ingredients. Gingival in vitro 3D models are commercially available.

The impact of airborne ultrafine particulate matter on human keratinocyte stem cells.

Objective: Air pollution is today fully acknowledged to be a significant public health problem. Rapid urbanization exposed us to a variety of unhealthy ambient air pollutants at high concentrations. The emergence of airborne ultrafine particles has added an additional dimension to this already complex problem of air pollution.

Reconstruction of functional human epidermis equivalent containing 5%IPS-derived keratinocytes treated with mitochondrial stimulating plant extracts.

Reconstructed human epidermis equivalents (RHE) have been developed as a clinical skin substitute and as the replacement for animal testing in both research and industry. KiPS, or keratinocytes derived from induced pluripotent stem cells (iPSCs) are frequently used to generate RHE. In this study, we focus on the mitochondrial performance of the KiPS derived from iPSCs obtained from two donors.

Glyoxal Induces Senescence in Human Keratinocytes through Oxidative Stress and Activation of the Protein Kinase B/FOXO3a/p27 Pathway.

Senescence is a well-characterized cellular state associated with specific markers such as permanent cell proliferation arrest and the secretion of messenger molecules by cells expressing the senescence-associated secretory phenotype. The senescence-associated secretory phenotype composition depends on many factors such as the cell type or the nature of the stress that induces senescence. Because the skin constitutes a barrier with the external environment, it is particularly subjected to different types of stresses and consequently prone to premature cellular aging.

Characterization of a New Full-Thickness Skin Model.

Since 30 years, bioengineering allowed to reconstruct human tissues using normal human cells. Skin is one of the first organ to be reconstructed thanks to the development of specific cell culture media and supports favoring the culture of human skin cells, such as fibroblasts, keratinocytes, or melanocytes. Skin models have evolved from epidermis to complex models including a dermis.

Establishment and performance assessment of an in-house 3D Reconstructed Human Cornea-Like Epithelium (RhCE) as a screening tool for the identification of liquid chemicals with potential eye hazard.

The objective of this project was to develop an in-house 3D Reconstructed Human Cornea-Like Epithelium (RhCE) to be used as a screening tool in early stages of product development. This study reports the establishment of the experimental procedure and the performance assessment of the model to discriminate liquid chemicals classified as causing serious eye damage/irritation (UN-GHS Category 1/2) from liquid chemicals not requiring such hazard classification (UN-GHS No Category). Histological examination of this ocular equivalent model, based on Normal Human Keratinocytes (NHK) cultured in a chemically defined medium, revealed a stratified and well-organized tissue construct.

Property characterization of reconstructed human epidermis equivalents, and performance as a skin irritation model.

In recent years, in vitro skin models combining cell biology and tissue engineering have been developed in order to replace animal models for toxicological studies and to serve as research support to better understand skin biology. This study reports the development and characterization of a epidermal tissue equivalent meant to be used to develop and to evaluate the effect of applied cosmetic ingredients, and for alternative toxicological testing. This epidermis equivalent model was characterized relative to the morphological characteristics of short- and long-term maintained tissues by performing histological studies.