Publications by authors named "Chrishan Fernando"
OLE (ornate, large, extremophilic) RNAs are members of a noncoding RNA class present in many Gram-positive, extremophilic bacteria. The large size, complex structure, and extensive sequence conservation of OLE RNAs are characteristics consistent with the hypothesis that they likely function as ribozymes. The OLE RNA representative from is known to localize to the phospholipid membrane and requires at least three essential protein partners: OapA, OapB, and OapC.
View Article and Find Full Text PDFOrnate, large, extremophilic (OLE) RNAs comprise a class of large noncoding RNAs in bacteria whose members form a membrane-associated ribonucleoprotein (RNP) complex. This complex facilitates cellular adaptation to diverse stresses such as exposure to cold, short-chain alcohols, and elevated Mg concentrations. Here, we report additional phenotypes exhibited by (formerly called ) strains lacking functional OLE RNP complexes.
View Article and Find Full Text PDFSeveral structured noncoding RNAs in bacteria are essential contributors to fundamental cellular processes. Thus, discoveries of additional ncRNA classes provide opportunities to uncover and explore biochemical mechanisms relevant to other major and potentially ancient processes. A candidate structured ncRNA named the " motif" has been found via bioinformatic analyses in over 2,500 bacterial species.
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- This study explored how primary caregivers in Australia accept a dental screening mHealth app designed for entering patient information and capturing dental photos to diagnose caries in preschool children.
- A questionnaire based on the Technology Acceptance Model was given to 42 caregivers, assessing factors like perceived ease of use, perceived usefulness, behavioral intention to adopt, anxiety, attitude, and self-efficacy.
- Results showed high satisfaction levels across most factors, indicating that perceived usefulness and positive attitudes significantly influenced caregivers' intentions to utilize the telehealth app for dental screenings, suggesting community readiness for its implementation.
Article Synopsis
- OLE RNA is a noncoding RNA found in many Gram-positive bacteria that live in extreme environments, such as high temperatures and salinity.
- It forms a ribonucleoprotein complex that is crucial for cell growth under stress, and disruption of this RNA or its protein partners leads to various growth issues.
- The authors suggest that the OLE RNP complex may function similarly to eukaryotic TOR complexes, helping bacteria adapt and coordinate essential cellular processes.
An R-loop is a three-stranded nucleic acid structure that consists of a DNA:RNA hybrid and a displaced strand of DNA. R-loops occur frequently in genomes and have significant physiological importance. They play vital roles in regulating gene expression, DNA replication, and DNA and histone modifications.
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