Publications by authors named "Chris Suarez"
Characterization of the exopolysaccharides produced by the industrial yeast Komagataella phaffii.
J Ind Microbiol Biotechnol
January 2024
Article Synopsis
- The yeast Komagataella phaffii is favored by biotech startups for producing recombinant proteins due to its safety record and effective production processes, but recent findings reveal a significant polysaccharide accumulation during fermentation that complicates product purity and adds processing costs.
- The study focused on using K. phaffii strain YB-4290 to produce lactoferrin, uncovering that a considerable amount of carbohydrate (mainly composed of mannose) co-purifies with the protein, indicating challenges in purification methods.
- Further investigations into carbohydrate sources showed that commonly used strains produce baseline exopolysaccharides, with higher levels occurring under recombinant protein expression, which may inform strategies for improving efficiency in protein production with K. ph
Understanding how members of the human gut microbiota prioritize nutrient resources is one component of a larger effort to decipher the mechanisms defining microbial community robustness and resiliency in health and disease. This knowledge is foundational for development of microbiota-directed therapeutics. To model how bacteria prioritize glycans in the gut, germfree mice were colonized with 13 human gut bacterial strains, including seven saccharolytic species.
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