Divergence of Rho residue 43 impacts GEF activity.

RhoA, RhoB and RhoC GTPases are over 85% identical at the amino acid level, with RhoA and RhoC differing at only one residue (43) across the initial two-thirds of their sequences. A putative regulatory distinction between the molecules is their capacity to be uniquely activated by guanine nucleotide exchange factors (GEFs). We hypothesize that variation of amino acid residue 43 between RhoA/B (valine) and RhoC (isoleucine) impacts GEF activity.

Use of bronchial blockers: a retrospective review of 302 cases.

The purpose of this retrospective review is to evaluate the safety and efficacy of the bronchial blockers (BBs) used in thoracic anesthesia. We enrolled 302 patients who had a BB placed to achieve one-lung ventilation (OLV). Variables recorded from the anesthetic record included type of device used, type and side of surgery, specific indications for OLV, Mallampati score, route of intubation, and complications related to the use of BBs.