Publications by authors named "Chris Finnis"
Article Synopsis
- Gene expression noise in yeast can lead to a mix of high- and low-producing cells, limiting overall metabolite production, which prompted this study to explore ways to enhance glutathione (GSH) output.
- Researchers engineered a counter-selection system in yeast that uses feedback inhibition to target low GSH-producing cells by linking GSH1 gene expression with a counter-selectable marker.
- This approach resulted in an 18% increase in mean GSH levels while ensuring the selected high-producing phenotype was non-heritable, demonstrating the potential to improve metabolite production by favoring high-performing cells in mixed populations.
Structures of native human thymidine phosphorylase and in complex with 5-iodouracil.
Eirini Mitsiki Anastassios C Papageorgiou Shalini Iyer Nethaji Thiyagarajan Steven H Prior Chris Finnis
Biochem Biophys Res Commun
September 2009
Thymidine phosphorylase (TP) first identified as platelet derived endothelial cell growth factor (PD-ECGF) plays a key role in nucleoside metabolism. Human TP (hTP) is implicated in angiogenesis and is overexpressed in several solid tumors. Here, we report the crystal structures of recombinant hTP and its complex with a substrate 5-iodouracil (5IUR) at 3.
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