A mathematical model to predict the optimal test line location and sample volume for lateral flow immunoassays.

Lateral flow immunoassay (LFIA) platform is one of the most relevant technologies for screening and diagnosing clinical conditions. However due to low sensitivity and poor repeatability of the platform it has been used only for limited and non-critical tests. Mathematical models have been used to understand the principles of capillary flow and antibody antigen based immunoreactions in nitrocellulose membrane typically seen in LFIA.