Chromosome constitution of equal-sized three-cell embryos using next-generation sequencing technology.

Purpose: To study the chromosome constitution of equal-sized three-cell embryo.

Methods: We determined the chromosome constitution of 105 blastomeres from 35 embryos using multiple annealing and looping-based amplification cycles (MALBAC) together with NGS sequencing technology. Chromosomal copy number variation (CNV) analysis was successfully performed in 27 embryos.