Publications by authors named "Chongqing Ma"

Article Synopsis
  • Extracellular outer-membrane vesicles (OMVs) from E. coli may be useful as drug nanocarriers due to their biocompatibility and cell entry ability, but low yields hinder their use.
  • A new method for harvesting OMVs using magnetic iron-oxide nanoparticles (MNPs) significantly increases yields, especially when MNPs are PEGylated, enhancing their uptake and secretion in E. coli.
  • Magnetically-harvested OMVs can be effectively targeted to specific locations in the human body and penetrate deeper into infectious biofilms compared to OMVs collected with traditional ultracentrifugation methods.
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Article Synopsis
  • Malignant tumors, like glioblastomas, can be targeted by cutting off their energy supply through metabolic reprogramming, which makes them more vulnerable to treatment.
  • Researchers developed a new platform, GLMSD, combining laser technology with drug screening to discover inhibitors specifically targeting hexokinase-2, a key enzyme in glycolysis, leading to the identification of five potential new drugs.
  • The most effective inhibitor, Compd 27, showed promising results in regulating metabolism and suppressing glioma tumor growth, especially when combined with an existing treatment, temozolomide, suggesting a pathway towards personalized cancer therapy.
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It is a pre-requisite to ionize analyte molecules efficiently for detection by laser desorption/ionization mass spectrometry. Here, we report a conceptual demonstration of cationizing neutral small molecules which are typically difficult to be ionized with the traditional organic matrices due to their low proton/cation affinity values. Our strategy features generating radical cations from site-specifically carboxylated 10-(4-carboxyphenyl)-10-phenothiazine-3,7-dicarboxylic acid (PTZ(A)-Ph(A)) with a laser, and anchoring the chlorine ion from NaCl through covalent bond-like bridging interactions with the N/S atoms in the heterocyclic structure.

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Gram-positive bacteria are one of the most common pathogens causing severe and acute infection, and hospital infection caused by Gram-positive bacteria have increased significantly. Also, as antibiotics have been widely used, abusing of antibiotics is becoming an increasingly serious problem which is followed by dangerous drug resistance. Here, we developed a series of cationic carbon dots (CDs) with high-performance as antibacterial agents by using tartaric acid and m-aminophenol as precursors.

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Appropriate prescription of antibiotics requires the pharmacokinetic knowledge of the drugs and their metabolites in blood, and their distribution/retention in organ tissues. Here we report that highly crystalline graphite dots (GDs) allow for quantitative profiling of antibiotic metabolites in a spatial-temporal manner, in combination with matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI). GDs matrix features an ultra-clean background base line and high efficiency in ionization of small molecules, thus enabling quantification of sulfamethoxazole (SMZ) and its metabolites with limit of detection (LOD) in the femtomole range.

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There is increasing demand for anti-doping drug monitoring in sports and food safety checks by developing sensitive and fast analytical methods. Here we report the development of hybrid Ir/SiNW as a new MALDI matrix for the detection of small molecules. This matrix is characterized by sufficient UV absorption, low-noise background, and high efficiency in ionization of small molecules.

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There is a great need to develop sensitive and specific methods for quantitative analysis of caspase-3 activities in cell apoptosis. Herein, we report a new method for sensitive detection of caspase-3 enzyme activities and inhibitor screening based on dual maleimide (DuMal) labeling quantitation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Evaluation of caspase-3 activities is performed using MS analysis of the enzymatic product of the peptide probe, which fuses a caspase-3 cleavable peptide segment (DEVD) and a quantifiable "ID tag" (a peptide segment of FRGLRGFKC labeled by maleimide).

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