Prediction of the molecular mechanism of eucommiae Cortex - Achyranthis bidentatae radix in the treatment of osteoarthritis: network pharmacology and molecular docking.

Objective: To retrieve the core drug of osteoarthritis in clinic using Data Mining, predict the drug molecular action target through the Network Pharmacology, identify the key nodes of the interaction by combining with the related targtes of osteoarthritis, explore the pharmacological mechanism of Traditional Chinese Medicine against osteoarthritis and other possible mechanisms of actions.

Methods: to retrieve the commonly used therapeutic formulations for osteoarthritis patients in clinical with PubMed, CNKI, VIP, CBM, Wan Fang Database and other databases, and screen out the core drugs through the Ancient and Modern Medical Case Cloud Platform and software Gephi, filter out the core drug molecules and targets combined with TCMSP database and the targets of osteoarthritis in Genecard and OMIM database, plunge those data into R project and Cytoscape to construct the intersection model of Drug molecule-osteoarthritis, establish PPI network and GO and conduct KEGG enrichment analysis with String database. Vina molecular docking was finally implemented to draw molecular docking diagram, and the results were analyzed after comprehensive analysis.

Chitosan-Based Surface Molecularly Imprinted Polymer Microspheres for Sustained Release of Sinomenine Hydrochloride in Aqueous Media.

The surface molecular imprinting technique has been proposed as a prospective strategy for template molecule recognition and separation by devising the recognition sites on the surface of imprinted materials. The purpose of this study was to establish a novel drug delivery system which was developed by surface molecular imprinting method using β-cyclodextrin (β-CD)-grafted chitosan (CS) (CS-g-β-CD) microspheres as matrix and sinomenine hydrochloride (SM) as the template molecule. By adjusting the amount of functional monomer and cross-linking agent, we got the more excellent adsorption of CS-g-β-CD molecularly imprinted polymers (MIPs-CS-g-β-CD).

[Clinical effects of total knee arthroplasty in the treatment of ankylosed knee].

Objective: To investigate the clinical effects of total knee arthroplasty in treatment of ankylosed knee caused by various reasons.

Methods: Four patients with 7 ankylosed knees, caused by ankylosing spondylitis in 1 case, septic arthritis with bony ankylosis in 1 case, and rheumatoid arthritis in 2 cases, underwent artificial knee replacement. Before the operation joint activity was 0 degrees , Knee Society score (KSS) was 42 (11 - 63), and the function score was 17.

[Computer-assisted navigation for total knee arthroplasty: a comparative study with conventional methods].

Objective: To compare the effects of Computer-assisted navigation (CAS) and conventional location technique in total knee arthroplasty (TKA).

Methods: Eight patients (11 knees) underwent CAS and 9 patients (11 knees) underwent conventional location technique for TKA. Follow-up was conducted for 5 - 12 months.