Site-selective, reversible, pH-induced N-terminal maleylation and its application for proteomics research.

Rationale: Compared with traditional labelling reagents used in proteomics, maleic anhydride is milder and can be easily removed under certain conditions, thus simplifying chemical derivatization.

Methods: The proposed strategy combined a site-specific chemical labelling reaction with mass spectrometry. Site-selective, reversible N-terminal maleylation was controlled by pH.

Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: remarkable UVB resistance and efficient DNA damage repair.

Bacteria living in the Antarctic region have developed several adaptive features for growth and survival under extreme conditions. Chlamydomonas sp. ICE-Lis well adapted to high levels of solar UV radiation.

ERK1/2 and AKT are vital factors in regulation of the migration of rat Schwann cells.

After peripheral nerve injury, Wallerian degeneration (WD) occurs in the distal nerve segment. During the process of degeneration, Schwann cells (SCs) dedifferentiate, proliferate and migrate to align in "bands of Büngner", providing structural guidance and growth-promoting substrates to regenerating axons. The molecular signals that trigger SCs migration remain unclear.

[Genetic polymorphism of six short tandem repeat loci in the Han population in Hebei province of China].

Objective: This article reports a population genetic study on six short tandem repeat(STR) loci, D7S820, D19S253, D12S391, D5S818, D16S539 and D8S1179, in a sample of unrelated Chinese Han individuals(n=122-173) living in Hebei province.

Methods: DNA extraction from blood samples (200 in number) and multiplex amplification of the above six loci were carried out. Using denaturing polyacrylamide gel electrophoresis and silver stain, the authors investigated the distribution of allele frequencies of the six loci in Han population in Hebei province.