Sodium alginate and bovine serum albumin co-combined improved the cryopreservation quality of boar sperm through the PI3K-AKT pathway.

Context The occurrence of apoptosis due to transient oxidative damage to spermatozoa presents a significant challenge in semen preservation. At present, the addition of protective agents is the primary method for mitigating this damage and involves the addition of protective agents. Aims This study aims to elucidate the mechanism of action of cryopreservation and to identify high-quality combinatorial cryopreservation dilutions.