A stand-alone and point-of-care electrochemical immuno-device for Salmonella typhimurium testing.

The rapid development of accurate and point-of-care diagnostic tools for foodborne diseases has made a massive impact in global health. Salmonella typhimurium (S. typhimurium) exemplifies an enteric pathogen, being a gram-negative bacteria responsible for several gastrointestinal and systemic illnesses.

A fully integrated wireless microfluidic immunosensing system for portable monitoring of Staphylococcus aureus.

The advanced devices that function fully without the need for external accessories are regarded as a pinnacle goal in the design and construction of modern ones. Staphylococcus aureus (S. aureus), a prominent human pathogen, is responsible for causing a wide variety of infections and chronic diseases.

Flexible epidermal wearable sensor for Athlete's sweat biomarkers monitoring.

Wearable sweat sensors hold great promise for the monitoring of athletic sweat biomarkers that are reflective of physical status and the inimitable feature of wearable sensors to conduct dynamic sweat analysis in situ. However, the preparative methods of wearable patches for monitoring athlete's biomarkers are often complicated. Here, we demonstrate the first example of "sports lab-on-skin" as a fully integrated epidermal sweat sensor through simple laser engraving and laser cutting methods, which enables on-body and wirelessly measuring sweat Na, sweat K, sweat lactate, and initial sweat rate for physical status assessment.

Progress in systematics and biogeography of Orchidaceae.

Orchidaceae are one of the largest families of angiosperms in terms of species richness. In the last decade, numerous studies have delved into reconstructing the phylogenetic framework of Orchidaceae, leveraging data from plastid, mitochondrial and nuclear sources. These studies have provided new insights into the systematics, diversification and biogeography of Orchidaceae, establishing a robust foundation for future research.

Innovative Colorimetric NQO1 Detection Strategy via Substrate Competitive and Biomimetic Cascade Reactions with a Highly Active NADH Oxidase Mimic.

NAD(P)H: quinone oxidoreductase-1 (NQO1) plays critical roles in antioxidation and abnormally overexpresses in tumors. Developing a fast and sensitive method of monitoring NQO1 will greatly promote cancer diagnosis in clinical practice. This study introduces a transformative colorimetric detection strategy for NQO1, harnessing an innovative competitive substrate mechanism between NQO1 and a new NADH oxidase (NOX) mimic, cobalt-nitrogen-doped carbon nanozyme (CoNC).

Accelerated and precise identification of antioxidants and pesticides using a smartphone-based colorimetric sensor array.

The integration of smartphones with conventional analytical approaches plays a crucial role in enhancing on-site detection platforms for point-of-care testing. Here, we developed a simple, rapid, and efficient three-channel colorimetric sensor array, leveraging the peroxidase (POD)-like activity of polydopamine-decorated FeNi foam (PDFeNi foam), to identify antioxidants using both microplate readers and smartphones for signal readouts. The exceptional catalytic capacity of PDFeNi foam enabled the quick catalytic oxidation of three typical peroxidase substrates (TMB, OPD and 4-AT) within 3 min.

Electrochemical microfluidic sensing platforms for biosecurity analysis.

Biosecurity encompasses the health and safety of humans, animals, plants, and the environment. In this article, "biosecurity" is defined as encompassing the comprehensive aspects of human, animal, plant, and environmental safety. Reliable biosecurity testing technology is the key point for effectively assessing biosecurity risks and ensuring biosecurity.

A Fully Integrated Handheld Electrochemical Sensing Platform for Point-of-Care Testing of O157:H7.

Fully integrated devices that enable full functioning execution without or with minimum external accessories or equipment are deemed to be one of the most desirable and ultimate objectives for modern device design and construction. O157:H7 ( O157:H7) is often linked to outbreaks caused by contaminated water and food. However, the sensors that are currently used for point-of-care O157:H7 ( O157:H7) detection are often large and cumbersome.

 (Orchidaceae, Vandeae), a new species from Yunnan, China.

A new species of Orchidaceae, , from Xichou County, Yunnan, China, is described and illustrated. The novelty is close to , , and , but differs from them by having a distinct, fleshy anterior callus with a deeply lobed apex at the base of the labellum and lateral lobes of labellum reflexed and facing outward.

Artificial enzyme innovations in electrochemical devices: advancing wearable and portable sensing technologies.

With the rapid evolution of sensing technologies, the integration of nanoscale catalysts, particularly those mimicking enzymatic functions, into electrochemical devices has surfaced as a pivotal advancement. These catalysts, dubbed artificial enzymes, embody a blend of heightened sensitivity, selectivity, and durability, laying the groundwork for innovative applications in real-time health monitoring and environmental detection. This minireview penetrates into the fundamental principles of electrochemical sensing, elucidating the unique attributes that establish artificial enzymes as foundational elements in this field.

Seconds Timescale Synthesis of Highly Stretchable Antibacterial Hydrogel for Skin Wound Closure and Epidermal Strain Sensor.

Effective wound healing is critical for patient care, and the development of novel wound dressing materials that promote healing, prevent infection, and are user-friendly is of great importance, particularly in the context of point-of-care testing (POCT). This study reports the synthesis of a hydrogel material that can be produced in less than 10 s and possesses antibacterial activity against both gram-negative and gram-positive microorganisms, as well as the ability to inhibit the growth of eukaryotic cells, such as yeast. The hydrogel is formed wholly based on covalent-like hydrogen bonding interactions and exhibits excellent mechanical properties, with the ability to stretch up to more than 600% of its initial length.

The Sapria himalayana genome provides new insights into the lifestyle of endoparasitic plants.

Background: Sapria himalayana (Rafflesiaceae) is an endoparasitic plant characterized by a greatly reduced vegetative body and giant flowers; however, the mechanisms underlying its special lifestyle and greatly altered plant form remain unknown. To illustrate the evolution and adaptation of S. himalayasna, we report its de novo assembled genome and key insights into the molecular basis of its floral development, flowering time, fatty acid biosynthesis, and defense responses.

Dual targets-induced specific hemin/G-quadruplex assemblies for label-free electrochemical detection capable of distinguishing Salmonella and its common serotype in food samples.

Efficient detection of pathogenic bacteria is paramount for ensuring food safety and safeguarding public health. Herein, we developed a label-free and signal-on dual-target recognition electrochemical DNA sensing platform based on the conformational formation of split G-quadruplex. This platform focused on achieving sensitive and low-cost detection of Salmonella and its most human-infecting S.

 (Orchidaceae, Epidendroideae, Vandeae), a new species from Xizang, China.

A new species of Orchidaceae, , from Motuo, Xizang, is described and illustrated based on morphological characters and molecular phylogenetics analysis. Molecular phylogenetic analysis and morphological characters indicate that is close to , and , but differs from them by having triangular wings on the column foot, rhombic lip mid-lobe with a fleshy-horned appendage at the base, and concave lip lateral lobes, the lower part white with a deep purplish-red spot and hairy, the upper part pale yellow with dense rust spots.

The extremely reduced, diverged and reconfigured plastomes of the largest mycoheterotrophic orchid lineage.

Background: Plastomes of heterotrophic plants have been greatly altered in structure and gene content, owing to the relaxation of selection on photosynthesis-related genes. The orchid tribe Gastrodieae is the largest and probably the oldest mycoheterotrophic clade of the extant family Orchidaceae. To characterize plastome evolution across members of this key important mycoheterotrophic lineage, we sequenced and analyzed the plastomes of eleven Gastrodieae members, including representative species of two genera, as well as members of the sister group Nervilieae.

Guided Synthesis of a Mo/Zn Dual Single-Atom Nanozyme with Synergistic Effect and Peroxidase-like Activity.

We present a facile route towards a dual single-atom nanozyme composed of Zn and Mo, which utilizes the non-covalent nano-assembly of polyoxometalates, supramolecular coordination complexes as the metal-atom precursor, and a macroscopic amphiphilic aerogel as the supporting substrate. The dual single-atoms of Zn and Mo have a high content (1.5 and 7.

A Flexible Microfluidic Chip-Based Universal Fully Integrated Nanoelectronic System with Point-of-Care Raw Sweat, Tears, or Saliva Glucose Monitoring for Potential Noninvasive Glucose Management.

By combining the distinctive noninvasive feature with the peculiar complete functional implementation trait, fully integrated raw noninvasive biofluid glucose biosensors offer active and remote glucose monitoring while posing minimal harm or infection risks compared to the traditional invasive manner. However, each previously reported fully integrated raw noninvasive biofluid glucose biosensor is solely focused on single-type raw noninvasive biofluid analysis. Given the diversity and complexity of subjects' physical conditions, single-type raw noninvasive biofluids are inappropriate to all crowds (e.

Signal-On Electrochemical Detection for Drug-Resistant Hepatitis B Virus Mutants through Three-Way Junction Transduction and Exonuclease III-Assisted Catalyzed Hairpin Assembly.

The present detection method for hepatitis B virus (HBV) drug-resistant mutation has a high misdiagnosis rate and usually needs to meet stringent requirements for technology and equipment, leading to complex and time-consuming manipulation and drawback of high costs. Herein, with the purpose of developing cost-effective, highly efficient, and handy diagnosis for HBV drug-resistant mutants, we propose an electrochemical signal-on strategy through the three-way junction (3WJ) transduction and exonuclease III (Exo III)-assisted catalyzed hairpin assembly (CHA). To achieve single-copy gene detection, loop-mediated nucleic acid isothermal amplification (LAMP), one of the highly promising and compatible techniques to revolutionize point-of-care genetic detection, is first adopted for amplification.

Four new species and a new record of Orchidinae (Orchidaceae: Orchideae) from China.

Four new species of Orchidaceae from China, , , , and , together with a new country record, , are described and illustrated based on morphological and/or phylogenetic analyses. is closely related to but differs from it by having the dorsal sepal ovate-orbicular and lip mid-lobe distinctly shorter than lateral lobes. is close to but is distinguished from it by having several fascicled and straight, root-like tubers (vs.

Synthesis of a clustered carbon aerogel interconnected by carbon balls from the biomass of taros for construction of a multi-functional electrochemical sensor.

In this study, a clustered carbon aerogel interconnected by carbon balls (CCAI-CB) was prepared as an electrode material to construct a multi-functional electrochemical sensor. CCAI-CB derived from taros (Colocasia esculenta (L). Schott) possesses meso-macroporous structure and plenty of defective sites, and shows notable activity in electrocatalysis as an electrode material.

Sweet potato derived three-dimensional carbon aerogels with a hierarchical meso-macroporous and branching nanostructure for electroanalysis.

In this paper, sweet potatoes (Ipomoea batatas) are used as low-cost precursors to synthesize carbon aerogels with a hierarchical meso-macroporous and branching nanostructure (HMM-BNCA). An HMM-BNCA-modified glassy carbon electrode (GCE) (HMM-BNCA/GCE) exhibits high electrocatalytic activity for some electroactive biomolecules. For ascorbic acid (AA), the HMM-BNCA/GCE exhibits low oxidation peak potential and detection limit (-0.

A Bendable Biofuel Cell-Based Fully Integrated Biomedical Nanodevice for Point-of-Care Diagnosis of Scurvy.

Fully integrated nanodevices that allow the complete functional implementation without an external accessory or equipment are deemed to be one of the most ideal and ultimate goals for modern nanodevice design and construction. In this work, we demonstrate the first example of a bendable biofuel cell (BFC)-based fully integrated biomedical nanodevice with simple, palm-sized, easy-to-carry, pump-free, cost-saving, and easy-to-use features for the point-of-care (POC) diagnosis of scurvy from a single drop of untreated human serum (down to 0.2 μL) by integrating a bendable and disposable vitamin C/air microfluidic BFC (micro-BFC) (named iCard) for self-powered vitamin C biosensing with a custom mini digital LED voltmeter (named iBox) for signal processing and transmission, along with a ″built-in″ biocomputing BUFFER gate for intelligent diagnosis.

Biomass derived worm-like nitrogen-doped-carbon framework for trace determination of toxic heavy metal lead (II).

The worm-like nitrogen-doped-carbon framework (WNCF) with abundant edge-plane-like defective sites (EDSs) was synthesized by using natural wax gourd (Benincasa hispida) as the main carbon precursor and milk yielded by Chinese Holstein cattle (Holstein Friesian) as the nitrogen precursor for the first time. The Nafion-dispersed WNCF (Nafion-WNCF) was employed to design a highly sensitive electrochemical sensor for the trace determination of toxic heavy metal lead (II) (Pb) by the differential pulse anodic stripping voltammetry (DPASV). Some key experimental factors including calcination temperature of WNCF, pH value of the buffer solution, deposition potential, deposition time and the concentration of bismuth (Bi) were optimized for the stripping analysis of Pb.

Point-of-care assay for drunken driving with Pd@Pt core-shell nanoparticles-decorated ploy(vinyl alcohol) aerogel assisted by portable pressure meter.

Alcohol abuse causes health problems and security accidents. A reliable and sensitive detection system for alcohol has been an instinctive demand in law enforcement and forensic. More efforts are demanded in developing new sensing strategy preferably with portable and non-invasive traits for the pushforward of point-of-care (POC) device popularization.