Expression, localization, and clinical application of the RNA binding domain of U1-70kD in HEp-2 cells.

Objective: To develop an improved substrate for indirect immunofluorescent test (IIF) to detect anti-U1-70kD autoantibodies.

Methods: The RNA binding domain of U1-70kD (U1BD) complementary DNA was obtained from human larynx carcinoma cell line HEp-2 by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into the mammalian expression vector pEGFP-C1. The recombinant plasmid pEGFP-U1BD was transfected into HEp-2 cells.

Expression, localization and clinical application of exogenous Smith proteins D1 in gene transfected HEp-2 cells.

Aim: To establish an improved substrate for an indirect immunofluorescence test (IIF) to detect anti-Sm antibody.

Methods: Full-length Smith protein D1(Sm-D1) complementary DNA was obtained from human larynx carcinoma cell line HEp-2 by reverse transcription - polymerase chain reaction (RT-PCR) and cloned into the mammalian expression vector pEGFP-C1. The recombinant plasmid pEGFP-Sm-D1 was transfected into HEp-2 cells.