Production of a new tetravalent vaccine targeting fimbriae and enterotoxin of enterotoxigenic .

Enterotoxigenic (ETEC) is an important type of pathogenic bacteria that causes diarrhea in pigs. The objective of this study was to prepare a novel tetravalent vaccine to effectively prevent piglet diarrhea caused by In order to realize the production of tetravalent inactivated vaccine, the biological characteristics, stability, preservation conditions, and safety of the recombinant strain BL21(DE3) (pXKKSL4) were studied, and the vaccine efficacy and minimum immune dose were measured. The results indicated that the biological characteristics, target protein expression, and immunogenicity of the 1st to 10th generations of the strain were stable.

NPC1L1 Plays a Novel Role in Nonalcoholic Fatty Liver Disease.

Niemann-Pick C1-Like 1 (NPC1L1) is a key protein in the transport of cholesterol, which exists in the brush marginal membrane of the intestinal epithelial cells and the timid duct membrane of the liver. It affects cholesterol absorption and plasma low-density lipoprotein levels. Cholesterol is both an important component of the cell membrane and a precursor of bile acid and steroid hormone synthesis.

Preparation of novel trivalent vaccine against enterotoxigenic Escherichia coli for preventing newborn piglet diarrhea.

Objective: To develop a trivalent genetically engineered inactivated Escherichia coli vaccine (K88ac-3STa-LTB) that neutralizes the STa toxin by targeting fimbriae and entertoxins for the treatment of enterotoxigenic E coli.

Animals: 18- to 22-g mice, rabbits, pregnant sows.

Procedures: Using PCR, the K88ac gene and LTB gene were cloned separately from the template C83902 plasmid.

Plasma SCF/c-kit Levels in Patients with Dipper and Non-Dipper Hypertension.

Objective The aim of this study was to investigate the relationship between peripheral plasma stem cell factor (SCF)/c-kit levels and the types of dipper and non-dipper hypertension in hypertensive patients. Methods This cross-sectional study included newly diagnosed hypertensive patients who underwent 24-hour ambulatory blood pressure monitor (ABPM) between January 2009 and 2012 in Jiangning city. Patients were divided into the dipper group and the non-dipper group according to ABPM measurements.

[Expression of alpha-toxin gene of Clostridium perfringens type A and its primary immunological protective function].

Alpha-toxin gene was amplified from chromosomal DNA of Clostridium perfringens type A by polymerase chain reaction (PCR). PCR product was inserted into vector pGEM-T directly. The cloned recombinant plasmid pXCPA02 possesses positive nucleotide sequence of alpha-toxin.

[Fusion of betal-toxin gene and beta2-toxin gene from Clostridium perfringens type C].

Betal-toxin and beta2-toxin genes from chromosomal DNA of Clostridium perfringens type C were amplified by PCR, PCR products were cleaved with restriction endonucleases and recovered. The recombinant plasmid pETXB1-2 containing beta1-beta2 fusion genes was constructed by recombinant technique and then transformed into Escherichia coli BL21 (DE3). The beta1-beta2 fusion proteins were expressed in recombinant strain BL21 (DE3) (pETXB1-2), and the expression level of the beta1-beta2 fusion proteins was about 15.