Dynamic human exposure to airborne bacteria-associated antibiotic resistomes revealed by longitudinal personal monitoring data.

Airborne antibiotic-resistant bacteria (ARB) can critically impact human health. We performed resistome profiling of 283 personal airborne exposure samples from 15 participants spanning 890 days and 66 locations. We found a greater diversity and abundance of airborne bacteria community and antibiotic resistomes in spring than in winter, and temperature contributed largely to the difference.

[Difference of P content in different area substrate of constructed wetland].

Adsorption of substrate is the main removal mechanisms of phosphorus in constructed wetland. It is easily impacted by various environmental factors existing in the wetland bed. The contents of substrate TP and the main inorganic P in different areas of both horizontal sub-surface flow constructed wetland with plant and one without plant were measured after treating wastewater five months.

[Effects of allelochemical isolated from Phragmites communis on algal membrane permeability].

Efflux of K+, Mg2+, Ca2+ ions from algal cells as signals of cell membrane permeability, inductively coupled plasma mass spectrometry (ICP-MS) as detection method of ions, the present research investigated effects of allelochemical eathyl-2-methyl acetoacetate (EMA) isolated from Phragmites communis on cell membrane permeability of Microcystis aeruginosa, Chlorella pyrenoidosa and Chlorella vulagaris. The results showed that, when the cells were boiled for 10 min and the membrane was destroyed absolutely, the K+ efflux of M. aeruginosa and C.

[Effects of allelochemical EMA isolated from Phragmites communis on algal cell membrane lipid and ultrastructure].

In order to reveal the antialgal mechanisms of allelochemicals, effects of the allelochemical eathyl-2-methyl acetoacetate (EMA) on cell membrane lipid and ultrastructure of Chlorella pyrenoidosa, Microcystis aeruginosa and Chlorella vulagaris were studied in this paper. The lipid fatty acids of the algal membrane were isolated following the Bligh and Dye method and quantified by gas chromatograph/mass spectrometry. The ultrastructure of algal cells was observed with TEM.

[Hydrodynamic effects of the oxidation ditch on the removal efficiency and energy consumption].

The hydrodynamic characteristics in the oxidation ditch have great effects on the distributions of the dissolved oxygen and the velocity gradient, and then make important effects on the removal efficiency and energy consumption. The single-ditch Passver oxidation ditch with the capacity of 500 m3/d was investigated. The measurement and computer simulation were carried out on the velocity gradient and degrees, 180 degrees, respectively, which lied on the corner of the downriver of the aerator.

The unfolded protein response transducer Ire1p contains a nuclear localization sequence recognized by multiple beta importins.

The Ire1p transmembrane receptor kinase/endonuclease transduces the unfolded protein response (UPR) from the endoplasmic reticulum (ER) to the nucleus in Saccharomyces cerevisiae. In this study, we analyzed the capacity of a highly basic sequence in the linker region of Ire1p to function as a nuclear localization sequence (NLS) both in vivo and in vitro. This 18-residue sequence is capable of targeting green fluorescent protein to the nucleus of yeast cells in a process requiring proteins involved in the Ran GTPase cycle that facilitates nuclear import.

[Effect of environment and nutrient factors on the content of nitrogen and phosphorus in two duckweeds species: Spirodela polyrrhiza and Lemna aequinoctialis].

Effects of temperature, light density, nitrogen level and phosphorus level of medium to the content of two species Spirodela polyrrhiza and Lemna aequinoctialis were researched. The results showed that the content of nitrogen in two species kept constant when the concentration of nitrogen was higher than 3 mg x L(-1) and temperature higher than 25 degrees C. It decreased under lower concentrations of nitrogen and lower temperature.

[Growth feature of biomass of Lemna aequinoctialis and Spirodela polyrrhiza in medium with nutrient character of wastewater].

Duckweeds have an important potential in nutrient recovery from wastewater because of their rapid multiplication and high protein content in biomass. The growth rate of duckweed biomass has a direct relationship with nutrient removal and recovery. With laboratory experiments of batch culture and continuous culture, the growth curves of two duckweed species, Lemna aequinoctialis and Spirodela polyrrhiza, cultivated in different media were gotten and fitted by Logistic model.

Signal- and importin-dependent nuclear targeting of the kidney anion exchanger 1-binding protein kanadaptin.

Kanadaptin (kidney anion exchanger adaptor protein) has recently been identified as a protein with binding activity to the cytoplasmic domain of the kidney Na(+)-independent Cl(-)/HCO(-)(3) anion exchanger 1 (kAE1). Since it is widely expressed in tissues devoid of kAE1, however, kanadaptin is likely to have additional cellular roles. This is supported by its multidomain structure, and possession of three clusters of basic amino acids exhibiting similarity to known nuclear localization sequences (NLSs).