[Imported B3 genotype measles viruses were isolated from measles cases in the Chinese mainland].

We isolated and identified the genotypes and molecular characteristics of the imported B3 measles virus (MeV) in the Chinese mainland. The Vero/SLAM cell line was used to isolate the viruses. Reverse transcription-polymerase chain reaction was undertaken to amplify the 450 nucleotide acids of the 3-terminal of the nucleoprotein gene.

[Genetic characterization analysis on the first imported measles virus of genotype D8 in Chinese mainland].

This study analyzed the genetic characterization on first imported measles virus of genotype D8 in Chinese mainland. Serums were collected from the suspicious MV patients to detect IgM antibody in ELISA. Throat swabs were cultured in Vero/SLAM cell line to get measles virus isolates.

[Molecular epidemiological analysis of rubella virus isolates from 2001 to 2011 in Shanghai, China].

Throat swabs collected from patients whose serum was measles IgM negative and rubella IgM positive during 2001-2011 were used to conduct cell culture for rubella virus. After identification of cell culture with RT-PCR, nucleotide of gene E1 of rubella virus was amplified and sequenced, followed by molecular epidemiological analysis. A total of 31 rubella viruses were isolated from 60 throat swabs.

[Isolation identification and serological analysis on varicella-zoster virus from an outbreak in Shanghai in 2007].

Objective: To make serological analysis and identify the etiology of an outbreak of chickenpox in Zhabei District of Shanghai in 2007.

Methods: IgM and IgG of the paired serums were detected by ELISA. The collected herpes were inoculated into MRC-5 cells to get VZV isolations and identify them by VZV standard serums.

Human bocavirus in children suffering from acute lower respiratory tract infection in Beijing Children's Hospital.

Background: Human bocavirus (HBoV) is a parvovirus recently found to possibly cause respiratory tract disease in children and adults. This study investigated HBoV infection and its clinical characteristics in children younger than five years of age suffering from acute lower respiratory tract infection in Beijing Children's Hospital.

Methods: Nasopharyngeal aspirates were collected from children suffering from acute lower respiratory tract infection during the winters of 2004 to 2006 (from November through the following February).