Benzothioate Glycoside from a terrestrial Streptomyces sp. TBRC 11511 from Thailand.

A new benzothioate glycoside metabolite, phitsanoside A, together with its new and known derivatives were isolated from Streptomyces sp. TBRC 11511 collected from sediment of a dry evergreen forest located in Phitsanulok Province, Thailand. The structure elucidation of the new compound was interpreted on the basis of spectroscopic data analysis.

Antimicrobial and Cytotoxic Angucyclic Quinones from .

Eight new angucyclic quinones, miaosporones A to H (-), along with the previously described metabolites 8-hydroxy-3-methylbenz[]anthraquinone (), tetrangulol (), 5,6-dihydro-1,8-dihydroxy-3-methybenz[]anthracene-7,12-quinone (), and SF2315A (), were isolated from the terrestrial actinomycete TBRC 5172 obtained from sediment collected from the Huai Yang reservoir, Prachuap Khiri Khan Province, Thailand. The relative and absolute configurations of the new compounds were determined from analysis of NMR spectroscopic and X-ray crystallographic data. Miaosporone A exhibited antimalarial activity against K1 and antibacterial activity against with respective IC values of 2.

Marinoterpins A-C: Rare Linear Merosesterterpenoids from Marine-Derived Actinomycete Bacteria of the Family Streptomycetaceae.

The chemical examination of two undescribed marine actinobacteria has yielded three rare merosesterterpenoids, marinoterpins A-C (-, respectively). These compounds were isolated from the culture broth extracts of two marine-derived actinomycetes associated with the family Streptomycetaceae, (our strains were CNQ-253 and AJS-327). The structures of the new compounds were determined by extensive interpretation of 1D and 2D NMR, MS, and combined spectroscopic data.

Cytochalasans Act as Inhibitors of Biofilm Formation of Staphylococcus Aureus.

During the course of our ongoing work to discover new inhibitors of biofilm formation of from fungal sources, we observed biofilm inhibition by cytochalasans isolated from cultures of the ascomycete for the first time. Two new compounds were purified by a bioassay-guided fractionation procedure; their structures were elucidated subsequently by nuclear magnetic resonance (NMR) spectroscopy and high-resolution mass spectrometry (HR-MS). This unexpected finding prompted us to test further cytochalasans from other fungi and from commercial sources for comparison.

Actinoquinolines A and B, anti-inflammatory quinoline alkaloids from a marine-derived Streptomyces sp., strain CNP975.

Actinomycete bacteria of the common genus Streptomyces can be routinely isolated from shallow and deep ocean sediments. Although commonly considered a terrestrial genus, and most abundantly found in soil, Streptomyces strains are found that have distinct requirements for seawater and routinely do not show significant similarity, with terrestrial strains by 16S ribosomal DNA phylogenetic sequence comparisons. Our examination of the culture broth of a Streptomyces sp.

Assessing medium constituents for optimal heterologous production of anhydromevalonolactone in recombinant Aspergillus oryzae.

Anhydromevalonolactone (AMVL) is a bioactive natural product that arises from a molecular biology technique using Aspergillus oryzae as a heterologous host. AMVL has been used as a precursor for the synthesis of insect pest control reagents and has numerous applications in the biotechnological and medical industries. In this study, the Plackett-Burman Design and the Central Composite Design, which offer efficient and feasible approaches, were complemented to screen significant parameters and identify the optimal values for maximum AMVL production.

Saccharosporones A, B and C, cytotoxic antimalarial angucyclinones from Saccharopolyspora sp. BCC 21906.

Three new angucyclinones, saccharosporones A, B and C, together with (+)-ochromycinone, (+)-rubiginone B2, tetrangulol methyl ether and fujianmycin A, were obtained from fermentation of the terrestrial actinomycete of the genus Saccharopolyspora BCC 21906 isolated from a soil collected in Chanthaburi Province, Thailand. Structures of the new compounds and their relative configurations were assigned by NMR spectral data interpretation. Saccharosporones A and B exhibited antimalarial activity against Plasmodium falciparum K1 with IC50 values of 4.

Functional expression of a foreign gene in Aspergillus oryzae producing new pyrone compounds.

Fungi from the genus Xylaria produce a wide range of polyketides with diverse structures, which provide important sources for pharmaceutical agents. At least seven polyketide synthase (PKS) genes, including pksmt, were found in Xylaria sp. BCC 1067.

Bireticulol, a bioactive isocoumarin dimer from Streptomyces sp. BCC24731.

A new dimeric isocoumarin, bireticulol, was isolated from the terrestrial Streptomyces sp. and characterized as a 5-5' dimer of reticulol. In addition, reticulol and 8-hydroxy-6,7-dimethoxy-3-methyl isocoumarin, together with other known polyketides piericidin A, 2'-(2-hydroxyphenyl)-2,4'-bibenzoxazole-4-carboxylic acid methyl ester (UK-1) and 3-benzyl-4-hydroxy-5-methyldihydrofuran-2-one were also obtained.

Marineosins A and B, cytotoxic spiroaminals from a marine-derived actinomycete.

Two novel spiroaminals, marineosins A and B (1, 2), containing two pyrrole functionalities, were isolated from cultures of a marine sediment-derived actinomycete related to the genus Streptomyces. The marineosins, which appear to be derived from unknown modifications of prodigiosin-like pigment pathways, showed significant inhibition of human colon carcinoma (HCT-116) in an in vitro assay (IC50 = 0.5 microM for marineosin A) and selective activities in diverse cancer cell types.

Eurysterols A and B, cytotoxic and antifungal steroidal sulfates from a marine sponge of the genus Euryspongia.

Two new steroidal sulfates, eurysterols A (1) and B (2), were isolated from an undescribed marine sponge of the genus Euryspongia collected in Palau. The structures of the new compounds were assigned by NMR spectroscopic data interpretation. Compounds 1 and 2 showed cytotoxicity against human colon carcinoma (HCT-116) cells with IC50 values of 2.