Improvement of the recombinant phytase expression by intermittent feeding of glucose during the induction phase of methylotrophic yeast Pichia pastoris.

Purpose: For growth of methylotrophic yeast, glycerol is usually used as a carbon source. Glucose is used in some cases, but not widely consumed due to strong repressive effect on AOX1 promoter. However, glucose is still considered as a carbon source of choice since it has low production cost and guarantees growth rate comparable to glycerol.

Determination of prostatic fluid citrate concentration using peroxidase-like activity of a peroxotitanium complex.

There have been developed many kinds of methods for detecting citrate in body fluids since citrate is very important physiologically and biochemically. In particular, determination of citrate concentration in prostatic or seminal fluid is useful in early diagnosis of prostate cancer. Recently, a peroxotitanium complex prepared from titanium tetrachloride and hydrogen peroxide has been shown to have peroxidase-like activity which is greatly inhibited by some hydroxyalkanoic acids.

Immobilization of -1333 on the Waste Ore Particles for the Continuous Oxidation of Ferrous Iron.

Background: The biooxidation of ferrous iron has a great potential for the regeneration of ferric iron, in operations such as bioleaching, bioremediation. Many natural inorganic materials were investigated for use as supports immobilizing . The waste chalcopyrite is another natural inorganic material of which particles are easy to prepare from the leached out ore heaps and the source is abundant.