Publications by authors named "Chloe Baron"
Article Synopsis
- Hematopoietic stem cells are influenced by their surrounding niche of endothelial and mesenchymal stromal cells in bone marrow, which plays a crucial role in leukemia development.
- Researchers discovered that leukemic cells release a peptide called apelin, which causes changes in the niche cells that promote the progression of leukemia.
- Blocking apelin in leukemic zebrafish showed a reduction in disease symptoms, suggesting that targeting apelin could be a promising new treatment approach for leukemia.
Article Synopsis
- Dysregulation of TGFb-related signaling pathways is a common feature in melanoma, leading to unique gene expression patterns in advanced stages of the disease.
- Researchers identified a specific TGFb enhancer active in melanoma cells, revealing that it is not present in normal or early melanoma cells but shows distinct expression in advanced melanomas.
- The study suggests that chronic TGFb signaling alters macrophage behavior and emphasizes the importance of developing biomarkers to improve patient responses to TGFb inhibitors.
Article Synopsis
- The study revealed that melanoma only formed in zebrafish melanocytes lining internal organs, mirroring the conditions in human patients and highlighting a distinct chromatin structure compared to skin melanomas.
- The findings indicated that zebrafish internal melanocytes share gene expression patterns with human MMs, showing characteristics linked to increased metastasis and decreased response to immunotherapy, thereby providing a valuable model for developing new treatments for MM.
Article Synopsis
- * Researchers used single-cell RNA sequencing on around 70,000 zebrafish cells to map immune cell development and found significant cellular diversity, especially in juvenile and adult thymus.
- * The study identified different immune cell types, including B- and T-cells, and suggested the presence of a pre-B cell state, laying important groundwork for future studies in zebrafish immunology.
Article Synopsis
- * The results showed that the biphasic strategy led to significantly higher rates of total and usable blastocysts and an increased live birth rate.
- * Additionally, gene analysis indicated that the oxygen concentration influenced the expression of genes related to embryo development and implantation, suggesting a biological basis for the improved outcomes with the biphasic method.
Article Synopsis
- - The study analyzes the miRNome (microRNA profile) of endometrial samples during the implantation window to see if it can predict outcomes in Assisted Reproduction Technology (ART) for patients with repeated implantation failures (RIF).
- - It involves a discovery cohort of 20 patients to identify various miRNAs associated with receptivity and outcomes like early miscarriage or live birth, followed by a validation cohort of 103 RIF patients to confirm findings with RT-qPCR.
- - Ultimately, four specific miRNAs are significantly linked to endometrial receptivity and implantation failure, suggesting that analyzing these profiles could help predict pregnancy success and enhance ART effectiveness.
Article Synopsis
- The regulation of messenger RNA (mRNA) levels in mammalian cells can be affected by how quickly mRNA is made and broken down.
- Traditional methods measured these rates in bulk populations, but understanding them in dynamic situations like cell differentiation requires analyzing individual cells.
- A new method was developed to track both new and existing mRNA in thousands of single cells, uncovering important strategies that influence gene expression during the cell cycle and differentiation processes.
Unravelling cellular relationships during development and regeneration using genetic lineage tracing.
Nat Rev Mol Cell Biol
December 2019
Article Synopsis
- Tracking the lineage of single cells is crucial for understanding processes like embryonic development and stem cell differentiation.
- Traditional methods use fluorescent labels that allow for the identification of a limited number of cell clones, but newer techniques employ heritable DNA barcodes and next-gen sequencing to track more clones in complex tissues.
- The review explores methods for both prospective and retrospective lineage tracing, including the use of genetic manipulation techniques and analysis of single-cell mRNA-sequencing data to gain insights into developmental paths and cell fate decisions.
Article Synopsis
- Current molecular and cell biology research often uses fluorescence-activated cell sorting (FACS) to purify specific cell types, which usually requires labeling cells with antibodies or using fluorescent genetic constructs.
- Limitations in antibody availability and the challenges of genetic manipulation, especially in human tissues, highlight the need for a method to enrich cell types without prior knowledge of their markers.
- The proposed method, GateID, utilizes single-cell transcriptomics combined with FACS index sorting to purify cell types based on inherent cellular properties, achieving high purity in experiments conducted on zebrafish kidney and human pancreas cells without the need for specific antibodies.
Article Synopsis
- - PROK1 levels in individual follicular fluid (FF) have been identified as a promising biomarker to predict the competence of oocytes in IVF, with a strong correlation to follicular size.
- - A study conducted at the University Hospital of Grenoble with 69 infertile couples confirmed that higher PROK1 concentrations in FF are indicative of better embryo development potential, independent of other embryo growth indicators.
- - The research suggests that hormones like Follicle Stimulating Hormone (FSH) and hCG can enhance PROK1 secretion, potentially affecting oocyte quality through biological pathways linked to cAMP.
Article Synopsis
- Haematopoietic stem cells (HSCs) develop from haemogenic endothelial (HE) cells during the formation of intra-aortic haematopoietic clusters (IAHCs) in vertebrate embryos, but the mechanisms behind this process are not well understood.
- Researchers conducted single-cell RNA-sequencing on various types of cells isolated from mouse embryo aortas to identify key genes and transcription factors involved in the endothelial-to-haematopoietic transition (EHT) and IAHC maturation.
- This study offers a comprehensive resource for studying HSC generation in vivo, which could lead to advancements in producing customized HSCs for treating blood-related diseases.
Article Synopsis
- Embryonic development involves progenitor cells that give rise to all adult body cells, making it essential to understand their fate in various tissues.
- Tracking clonal history and cell identity at a single-cell level has been challenging, but advancements like ScarTrace allow researchers to analyze thousands of cells simultaneously.
- Using ScarTrace, significant findings were made in zebrafish, including how certain progenitors lead to specific cell types in organs like the kidney, eyes, and brain, offering insights into cellular origins and differentiation during development.
Article Synopsis
- The RNAi pathway in insects provides antiviral immunity through the production of virus-specific siRNAs, which are amplified via reverse transcription of viral RNA into viral DNA (vDNA).
- Our research shows that vDNA exists in both linear and circular forms, with circular vDNA (cvDNA) being effective in generating siRNAs that help protect against related viral infections.
- We discovered that defective viral genomes (DVGs) not only contribute to vDNA and cvDNA synthesis but also enhance the RNAi response against viruses, with the Dicer-2 protein regulating vDNA production in a unique way.