Publications by authors named "Chiyo Yanagi-Mizuochi"
Article Synopsis
- The FANTOM5 project mapped transcription initiation events in human and mouse genomes with high precision using CAGE technology and single-molecule sequencing.
- Over 3,000 diverse samples, including primary cells and tissues, were analyzed through a standardized process starting from RNA quality assessment to generating transcription initiation frequencies.
- The analysis identified around 200,000 (human) and 150,000 (mouse) non-overlapping peaks, enabling the annotation of both known and novel promoters and providing insights into transcriptional regulation in different cellular states.
In mice, hematopoietic cells home to bone marrow from fetal liver prenatally. To elucidate mechanisms underlying homing, we performed immunohistochemistry with the hematopoietic cell marker c-Kit, and observed c-Kit(+) cells localized inside muscle surrounding bone after 14.5 days post coitum.
View Article and Find Full Text PDFAlthough it is generally accepted that cellular differentiation requires changes to transcriptional networks, dynamic regulation of promoters and enhancers at specific sets of genes has not been previously studied en masse. Exploiting the fact that active promoters and enhancers are transcribed, we simultaneously measured their activity in 19 human and 14 mouse time courses covering a wide range of cell types and biological stimuli. Enhancer RNAs, then messenger RNAs encoding transcription factors, dominated the earliest responses.
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