Changes to the meiotic spindle and zona pellucida of mature mouse oocytes following different cryopreservation methods.

This study is to investigate the change of morphology of the meiotic spindle and the extent of zona hardening relating to the morphological survival and developmental competence of thawed oocytes. Four- to 8-week-old female mice (C57BL/6) primed with an intraperitoneal injection of pregnant mare's serum gonadotropin and human chorionic gonadotropin. Cryopreserved oocytes using two protocols: vitrificaton using ethylene glycol (EG) and slow freezing using propanediol (PROH).

Laparoscopic ovarian drilling with diathermy in the treatment of infertile women with polycystic ovaries.

Background: [corrected] To describe the effect of laparoscopic ovarian drilling with diathermy on the pregnancy outcome of infertile women with polycystic ovaries.

Methods: Between September 2000 and July 2001, 6 infertile patients with polycystic ovaries who visited the Kinmen County Hospital consented to participate in the study. Oligomenorrhea was the common clinical symptoms of these 6 patients whose pelvic sonogram revealed typical pattern of polycystic ovaries.