A silk fibroin composite film that can simultaneously scavenge and probe HO in situ was developed for possibly examining local concentrations of HO for biomedical applications. A multi-functional composite film (GDES) that consists of graphene oxide (G), a photothermally responsive element that was blended with polydopamine (PDA, D)/horseradish peroxidase (HRP, E) (or DE complex), and then GDE microaggregates were coated with silk fibroin (SF, S), a tyrosine-containing protein. At 37 °C, the HO-scavenging ability of a GDES film in solution at approximately 7.
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