An mpox quadrivalent mRNA vaccine elicits sustained and protective immunity in mice against lethal vaccinia virus challenge.

Assessing the long-term efficacy of MPXV vaccine candidates is crucial for the global response to the ongoing mpox epidemic. Built upon our previous study of the mpox quadrivalent mRNA vaccine, herein we reported that MPXV-1103 could elicit sustained humoral and cellular immunity in mice, including the induction of MPXV A35/B6/A29/M1-specific IgG antibodies, VACV neutralizing antibodies and activated cytotoxic CD8T cells, which provides 100% protection against lethal VACV challenge even at 280 days after the first vaccination. Our results provide critical insights for orthopoxvirus vaccine development.

A cross-language speech model for detection of Parkinson's disease.

Speech change is a biometric marker for Parkinson's disease (PD). However, evaluating speech variability across diverse languages is challenging. We aimed to develop a cross-language algorithm differentiating between PD patients and healthy controls using a Taiwanese and Korean speech data set.

An adenosine analog shows high antiviral potency against coronavirus and arenavirus mainly through an unusual base pairing mode.

By targeting the essential viral RNA-dependent RNA polymerase (RdRP), nucleoside analogs (NAs) have exhibited great potential in antiviral therapy for RNA virus-related diseases. However, most ribose-modified NAs do not present broad-spectrum features, likely due to differences in ribose-RdRP interactions across virus families. Here, we show that HNC-1664, an adenosine analog with modifications both in ribose and base, has broad-spectrum antiviral activity against positive-strand coronaviruses and negative-strand arenaviruses.

Long-Lasting Protection and Dose Optimization of MPXV Polyvalent Mpox mRNA Vaccines Against Lethal Vaccinia Virus Challenge in Mice.

The outbreak of clade II monkeypox virus (MPXV) and the additional outbreak in Central Africa of clade I virus from 2023 have attracted worldwide attention. The development of a scalable and effective vaccine against the ongoing epidemic of mpox is urgently needed. We previously constructed two bivalent MPXV mRNA vaccines, LBA (B6R-A29L) and LAM (A35R-M1R), and a quadrivalent mRNA vaccine, LBAAM (B6R-A35R-A29L-M1R).

A Novel Circular Delta-XBB15 RBD Dimeric Protein Subunit Vaccine Mediated by Split Intein Elicits an Immune Response and Protection Against Multiple SARS-CoV-2 Variants in Mice.

SARS-CoV-2 continues to mutate, leading to breakthrough infections. The development of new vaccine strategies to combat various strains is crucial. Protein cyclization can enhance thermal stability and may improve immunogenicity.

Community pharmacy service provision to adults with palliative care needs in their last year of life: a scoping review.

Introduction The provision of palliative care is central to primary health care delivery. In this setting, community pharmacies often act in a medication supply role, yet their broader involvement in supporting people in the last year of life is less well understood. Aim This study aimed to review the literature on community pharmacies supporting adults with palliative care needs in their last year of life, emphasising challenges to optimising their role and improving equity and service accessibility.

Improving the prediction of patient survival with the aid of residual convolutional neural network (ResNet) in colorectal cancer with unresectable liver metastases treated with bevacizumab-based chemotherapy.

Background: To verify overall survival predictions made with residual convolutional neural network-determined morphological response (ResNet-MR) in patients with unresectable synchronous liver-only metastatic colorectal cancer (mCRC) treated with bevacizumab-based chemotherapy (BBC).

Methods: A retrospective review of liver-only mCRC patients treated with BBC from December 2011 to Apr 2021 was performed. Patients who had metachronous liver metastases or received locoregional treatment before the initiation of BBC were excluded.

Relationship Between Left Ventricular Ejection Fraction and ICD-10 Codes Among Patients Hospitalized With Heart Failure.

Introduction: While left ventricular ejection fraction (LVEF) represents an important means by which to classify patients with heart failure (HF), relatively little is known about the distribution of LVEFs among patients hospitalized for HF based on their International Classification of Disease (ICD)-10 code.

Methods: We performed a retrospective cross-sectional analysis of patients admitted to a large integrated health system within the western US between January 1, 2018 and October 1, 2022 with a principal diagnosis of HF (defined by ICD-10 codes: I50.2, systolic HF; I50.

Conventional Synthetic Disease-modifying Drugs Remain the Mainstay of Therapy for Rheumatoid Arthritis in India.

Background: There are limited data on the real-world utilization of disease-modifying antirheumatic drugs (DMARDs) in Indian patients with rheumatoid arthritis (RA).

Methods: This was a cross-sectional study of a multicentric observational cohort of RA patients across rheumatology clinics at six centers across India. Patients who met the American College of Rheumatology (ACR) 2010 criteria for RA were included.

Quit attempts, use of smoking cessation treatments and quitting intention among treatment seekers in rural areas.

Introduction: Rural populations have higher rates of tobacco use but are less likely to use evidence-based smoking cessation treatments than urban residents. There is limited evidence on the use of smoking cessation treatments and the factors associated with their use in rural populations. This study explored quit attempts, use of smoking cessation treatments, quitting intention and associated factors among rural residents seeking smoking cessation treatment.

Individualized Prediction for Risk of Recurrence in Stage I/II Melanoma Patients With Negative Sentinel Lymph Node.

Background: Despite the favorable prognosis of AJCC stage I/II melanoma patients, up to 20%-30% will develop metastases. Our objective is to predict long-term risk (probability) of recurrence in early-stage melanoma patients.

Methods: A Risk Score to predict long-term recurrence was developed using Cox regression based on 2668 patients.

Deposition of VS2/MoS2 bilayer layers of 2D material on nickel inverse opal structural substrates by SILAR and ECD processes as supercapacitor electrodes.

The composition, microstructure, and electrochemical properties of the two kinds of thin film electrode materials, namely VS2/MoS2/Ni-IOS and VS2/MoS2/ Ni-foam, were analyzed. The research results indicate that the self-assembled photonic crystal templates with adjusted EPSA processing parameters (100 V/cm; 7 min) would lead to an FCC closest-packed structure. Metallic nickel inverse opal structure (IOS) photonic crystals whose thickness can be freely regulated simply by electrochemical deposition time.

Effectiveness and safety of low-energy shock wave therapy for digital ulcers associated with systemic sclerosis: a phase 3 pivotal clinical trial.

Objectives: Systemic sclerosis is characterised by ischaemic skin ulcers on the fingertips, and low-energy shock wave therapy is suggested as a novel treatment for ischaemic lesions with angiogenic effects. We aimed to investigate the efficacy and safety of shock wave therapy for skin ulcers in patients with systemic sclerosis.

Methods: In this phase 3 pivotal study, we analysed 60 systemic sclerosis patients with digital ulcers that did not disappear after >4 weeks of existing treatment: 30 patients were treated with extracorporeal shock wave therapy and 30 with conventional treatment.

Unicompartmental Knee Arthroplasty Versus Opening-Wedge High Tibial Osteotomy for Spontaneous Osteonecrosis of the Knee: A Retrospective Cohort Study.

Background: Unicompartmental knee arthroplasty (UKA) and high tibial osteotomy (HTO) are treatment options for patients with medial spontaneous osteonecrosis of the knee (SONK).

Purpose: To compare the clinical outcomes after UKA and HTO in patients with SONK.

Study Design: Cohort study; Level of evidence, 3.

Twin atrioventricular nodes and accessory pathways in congenital heart diseases with abnormal atrioventricular connections: Association with the developmental hierarchy of cardiac morphology.

Background: Twin atrioventricular (AV) nodes (TWAVNs) are common in heterotaxy syndrome.

Objective: The purpose of this study was to investigate the presence and implications of TWAVNs and accessory pathways in congenital heart diseases (CHDs) with abnormal AV connections.

Methods: A retrospective study of a 1980-2022 cohort with sufficient electrocardiographic (ECG) data for review was conducted.

Graphene-supported organoiridium clusters catalyze -alkylation of amines hydrogen borrowing reaction.

Graphene-supported organic iridium clusters (GSOICs) have been designed, prepared, characterized, and used for -alkylation of amines hydrogen borrowing reactions. Structural analysis data (including IR, XPS, TEM and EDS) show that organoiridium clusters are uniformly formed on the surface of graphene, and the grain size of GSOIC is between 1 and 3 nm. After being activated by the auxiliary ligand TMPP (tris(4-methoxyphenyl)phosphine), GSOIC showed excellent catalytic performance for hydrogen borrowing reaction, with its turnover frequency (TOF) reaching 13.

The packaging signal of Xanthomonas integrative filamentous phages.

Unlike Ff, the packaging signal (PS) and the mechanism of integrative filamentous phage assembly remains largely unknown. Here we revived two Inoviridae prophage sequences, ϕLf2 and ϕLf-UK, as infectious virions that lysogenize black rot pathogen Xanthomonas campestris pv. campestris.

Perceived stress and betel quid dependence associated with reasons for betel quid chewing among male chewers: a mixed methods study.

Aims: Social culture concerns and betel quid (BQ) dependence may be the potential reasons for BQ chewing. This study's purpose was to identify the correlates of reasons for male BQ chewing.

Methods: We adopted a mixed-methods design.

Modulating magnetic anisotropy in linear tetranuclear dysprosium(III) complexes coordinated anions.

We report the synthesis, structures, and magnetic properties of two novel linear tetranuclear complexes with the general formula [Dy(Hheb)(heb)X(MeOH)] (X = NO, OAc; Hheb = ()-'-(1-(2-hydroxyphenyl)ethylidene)benzohydrazide, OAc = acetate). The rigid ligands (Hheb/heb) incorporate phenoxide groups and bridge the Dy ions in an unusual tetranuclear linear assembly. Notably, we demonstrate through magnetic measurements and theoretical calculations how the anion (X) coordinated at the peripheral Dy centers acts as a switch, significantly changing the magnetic anisotropy of the entire complex.

Abstract Versus Concrete Risk Identification in Clinical Research in Japan: Randomized and Prospective Pilot Research on the Effect of Risk Reduction Activities in a Risk-Based Approach.

Background: The risk-based approach (RBA) of clinical trial was first introduced in 2011-2012. RBA necessitates implementing risk reduction activities that are proportionate to risk in order to reduce avoidable quality issues. However, there is no consistent methodology or research for identifying and evaluating risks and planning risk reduction activities.

Childhood Maltreatment Associated with Anxiety and Depression and Complex PTSD Symptoms.

Background: Childhood maltreatment has great impact on physical and mental health. This study was designed to investigate the relationship between childhood maltreatment experience, social support, Anxiety and Depression, and traumatic stress symptoms in adults.

Methods: There were 113 subjects aged 20-35 recruited.