Publications by authors named "Chitra Sambare"
Prevalence and Predictors of Diabetic Retinopathy, Its Progression and Regression in Indian Children and Youth With Type-1 Diabetes.
Clin Med Insights Endocrinol Diabetes
September 2024
Article Synopsis
- The study aimed to investigate the prevalence of diabetic retinopathy (DR) in Indian children and youth with type-1 diabetes (T1D) and assess the effectiveness of screening recommendations.
- In a sample of 882 participants, the study found a DR prevalence of 6.4%, with all cases being early non-proliferative, and identified hypertension and older age as significant predictors.
- The results indicate a need for early screening of DR in T1D patients, as the condition is present even in younger individuals, emphasizing the importance of monitoring their health closely.
Purpose: The purpose of this study was to genotype two previously identified SNPs (rs1048661:R141L, and rs3825942:G153D) in the lysyl oxidase-like 1 (LOXL1) gene and determine their association with pseudoexfoliation glaucoma (XFG) in patients from Pune, India.
Methods: All subjects underwent detailed phenotyping, and DNA extraction was performed on blood samples by using standardized techniques. Exon 1 of the LOXL1 gene containing the SNPs (rs3825942:G153D; rs1048661:R141L) were Sanger sequenced, and the results were analyzed using sequence analysis software SeqScape 2.
Article Synopsis
- The study focused on COVID-19-associated rhino-orbital-cerebral mucormycosis (ROCM) in India, examining patient demographics, risk factors, symptoms, and management outcomes during the pandemic's second wave.
- Key findings revealed that 78% of patients had diabetes and a majority required oxygen support; most cases showed ROCM symptoms within 10-15 days post-COVID diagnosis.
- Final outcomes indicated a 14% mortality rate, significantly reduced through surgical interventions, highlighting the role of corticosteroids and diabetes as major contributors to ROCM development.
Purpose: The aim of this study was to determine whether mutations in mitochondrial DNA play a role in high-pressure primary open-angle glaucoma (OMIM 137760) by analyzing new data from massively parallel sequencing of mitochondrial DNA.
Methods: Glaucoma patients with high-tension primary open-angle glaucoma and ethnically matched and age-matched control subjects without glaucoma were recruited. The entire human mitochondrial genome was amplified in two overlapping fragments by long-range polymerase chain reaction and used as a template for massively parallel sequencing on an Ion Torrent Personal Genome Machine.
Intraocular lens power (IOL) calculation for cataract surgery has been shown to be inaccurate after photorefractive keratectomy (PRK), laser-assisted subepithelial keratectomy (LASEK) and laser in situ keratomileusis (LASIK). Many techniques exist to calculate corneal power with varying results and require the clinician to be aware of the pitfalls of IOL power calculation in post-refractive eyes. The AS biometry method proposed here is a simple method which does not rely on the calculation of corneal power.
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