Exploration of the candidate beneficial bacteria for Penaeus vannamei culture by core microbiome analysis using amplicon sequencing.

Globally, Penaeus vannamei is the vital species in aquaculture production. Beneficial bacterial exploration of gut, sediment, and water were investigated in P. vannamei culture using Illumina Miseq sequencing of 16S RNA V3-V4 hypervariable regions.

Comparative phylogenomics of Trueperella pyogenes reveals host-based distinction of strains.

Trueperella pyogenes, an opportunistic pathogen causes various ailments in different animals. Different strains from different animals have distinct characters phenotypically and genotypically. Hence understanding the strains in a particular geographical location helps in framing the preventive measures.

First report of the whole genome of Moraxella bovoculi genotype 1 from India and comparative genomics of Moraxella bovoculi to identify genotype-specific markers.

Moraxella bovoculi has been isolated frequently from cattle with Infectious bovine keratoconjunctivitis (IBK). Two diverse genotypes of M. bovoculi, 1 and 2 were identified based on whole genome sequence analysis.

A comparative analysis of biomass and clean fuel exposure on pulmonary function during cooking among rural women in Tamilnadu, India.

It is of interest to document data on the comparative analysis of biomass and clean fuel exposure on pulmonary function during cooking among rural women. The study consisted of 100 biomass and 100 LPG fuel using women with no smoking habits and other related illness Parameters such as FVC, FEV1, FEV1/FVC, PEFR, FEF25-75%were obtained using the computerized spirometry to assess the pulmonary function in these subjects. The collected data were analyzed using the Student t-test method and Pearson correlation.

Virulence genes and enterobacterial repetitive intergenic consensus region (ERIC) profiling reveals highly diverse genetic population among avian strains of Pasteurella multocida.

Pasteurella multocida is a multispecies pathogen with certain host specific capsular types but interspecies transmission cannot be overlooked. Knowing the diversity of P. multocida in a geographical location is essential to formulate a vaccination programme.

A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells.

Purpose: McCarey-Kaufman's (MK) medium and Optisol-GS medium are the most commonly employed media for human donor corneal preservation. In this study, we evaluated the preservation efficacy of discarded human donor corneas using a Thermo-reversible gelation polymer (TGP) added to these two media.

Methods: Thirteen human corneal buttons collected from deceased donors, which were otherwise discarded due to low endothelial cell density (ECD) were used.

Nitric Oxide Inhibition Assay and the Respective Target Identification of an Aptamer Designed to Control Atherosclerosis.

Introduction: Aptamers are emerging newer therapeutics and diagnostics whichcan be designed to bind any kind of target proteins. Vascular endothelial damage by the excess amount of nitric oxide production in systemic circulation leads to the secretion of inflammatory chemoattractants and cell adhesions are the prime pro-atherogenic events in the formation of plagues at atrial intimal layers due to oxidation - sensitive mechanisms. Nitric oxide inhibition assay is one of the valuable qualitative anti-atherosclerosis matrices.

Biosafety Concerns During the Collection, Transportation, and Processing of COVID-19 Samples for Diagnosis.

The coronavirus disease 2019 (COVID-19) pandemic, which started in China, has created a panic among the general public and health care/laboratory workers. Thus far, there is no medication or vaccine to prevent and control the spread of COVID-19. As the virus is airborne and transmitted through droplets, there has been significant demand for face masks and other personal protective equipment to prevent the spread of infection.

infection in sheep and goats in Delta region of Tamil Nadu.

Aim: The study was conducted to isolate and identify (DC) using conventional and molecular diagnostic techniques in scab materials collected from skin infections of sheep and goats in the Delta region of Tamil Nadu.

Materials And Methods: A total of 20 scab samples collected from 18 goats and 2 sheep from Nagapattinam, Thanjavur, and Tiruvarur districts of Tamil Nadu. Smears were made from softened scab materials and stained by either Gram's or Giemsa staining.

A rough set based rational clustering framework for determining correlated genes.

Cluster analysis plays a foremost role in identifying groups of genes that show similar behavior under a set of experimental conditions. Several clustering algorithms have been proposed for identifying gene behaviors and to understand their significance. The principal aim of this work is to develop an intelligent rough clustering technique, which will efficiently remove the irrelevant dimensions in a high-dimensional space and obtain appropriate meaningful clusters.

Detection and sequence analysis of accessory gene regulator genes of Staphylococcus pseudintermedius isolates.

Background: Staphylococcus pseudintermedius (SP) is the major pathogenic species of dogs involved in a wide variety of skin and soft tissue infections. The accessory gene regulator (agr) locus of Staphylococcus aureus has been extensively studied, and it influences the expression of many virulence genes. It encodes a two-component signal transduction system that leads to down-regulation of surface proteins and up-regulation of secreted proteins during in vitro growth of S.

Effect of processing paddy on digestibility of rice starch by in vitro studies.

Paddy (Oryza sativa L) (variety 'IR - 64'), was parboiled, puffed by sand roasting and flaked by edge runner and roller flaker and variations in physical and physicochemical properties were studied. Moisture contents were lower (5.8-10.

A pharmacognostical report on the rhizome of Alpinia galanga Linn. (Willd).

Alpinia galanga Linn. (Willd) is an important raw drug used in large quantities in various indigenous formulations. In this paper the major diagnostic characters and the qualitative chemical and physical tests responsible for the pharmacognostic identity of the rhizomes have been reported.

Radical scavenging activity of Trianthema triquetra in male albino rats intoxicated with CCl4.

Radical scavenging activity of ethanolic extract of Trianthema triquetra root was investigated against CCl4 in rats. The rats were treated with T. triquetra (100 mg, 200 mg/kg b.

Bioefficiency of indigogera tinctoria linn. On isoniazid induced hepatotoxicity in albinorats.

Indigofera tincoria leaf extract has been evaluated for its anti-hepatotoxic activity on Rats. Isoniazid is a first line agent for treatment of tuberculosis. Acute ingestion by adults with little dose can lead to mild liver toxicity.

Antibacterial activity of embelin.

Embelin, a benzoquinone-derivative isolated from Embelia ribes, when tested for its antibacterial potential exhibited significant inhibition against five and moderate activity against three strains of the 12 bacteria tested.

Pharmacognostical and phytochemical studies on curcuma amada (linn.) rhizome (zingiberaceae).

The macroscopic and microscopic characters, physical constant values, extractive values, ash values and the behaviour of powder drug on treatment with different chemical regents, microchemical and histochemical analyses were conducted to characterize some pharmacognostical parameters of Curcuma amada linn. (Zingiberaceae).

Pharmacognostical and phytochemical investifations on the tuberous roots of hemidesmus indicus (linn.) R.br. (Asclepiadaceae).

The macroscopic and microscopic characters, physical constant values, extractive values, ash values the behaviour of the powerdered drug on treatment with different chemical reagents, microchemical ad histochemical analyses were conducted to characterize some pharmacognostical parameters of Hemidesmus indicus (Linn) R,Br. (Asclepiadaceae).

[3H]-thymidine uptake and lipid peroxidation by tumor cells on embelin treatment: an in vitro study.

Using a rapid technique to assess drug-induced cell toxicity, a fibrosarcoma cell line was exposed in vitro to increasing concentrations of embelin, a benzoquinone derivative of Embelia ribes Burm., and simultaneously inoculated with [3H]-thymidine. After regular time intervals, the cells were examined for incorporation of the labelled thymidine in DNA, lipid peroxide and glutathione levels.

Antitumor, anti-inflammatory and analgesic property of embelin, a plant product.

Embelin, a plant-based benzoquinone derivative, has been found to exhibit significant antitumor activity in methylcholanthrene-induced fibrosarcoma in albino rats besides enhancing their survival time. The drug also has an appreciable action on pain and inflammation. The changes in DNA, RNA and protein levels in various organs in the tumor-bearing control and the drug-treated animals were also studied.

Interaction of human serum lipoproteins with biomaterials.

Human serum was incubated with representative portions of polyvinyl chloride (PVC) blood storage bags and vascular prostheses. The in vitro interaction process with lipoprotein was followed by polyacrylamide gel electrophoresis (PAGE) using sudan black and nitroblue tetrazolium (NBT) in the prestaining procedure. Densitometric scan of all the lipoprotein bands in serum after incubation with PVC bag material when prestained with sudan black showed remarkable increase in intensity.