[Influence of human fetal mesenchymal stem cells on glioma cell proliferation. A consequence of cellular crosstalk].

The effect of mesenchymal stem cells (MSCs) on the growth of various tumors is ambiguous. MSCs derived from different tissues stimulate growth of some tumor types and exert antitumor effects on the other. Several recent reports have shown that crosstalk between tumor cells and MSCs contribute to these effects.

[Some aspects of functional diagnostics in sportsmen of high qualification].

The article presents characteristics and prevalence of EKG changes among sportsmen of high qualification. A new interpretation of such changes is given in the article. EKG data had been compared with the dynamics of sports results.

[Quantitative analysis of morphogenesis of local nerve plexuses forming in tissue culture].

Discrete stages of formation of nervous plexuses in the culture of isolated neurons of a mollusc were analyzed autonomically. Area of plexus expansion (S1) and area, occupied by nerve structures (neurites and neuron bodies) were determined. It was demonstrated that despite intensive radial growth of peripheral neurites and S1 growth the density of plexus (S2/S1) was not changed.

[PCR diagnosis of sequences of a novel human herpes virus type 8 in patients with Kaposi sarcoma in Russia].

Associations of a new human herpesvirus type 8 (HHV-8) with different forms of Kaposi's sarcoma (KS) in Russia have been studied. Search for this virus genetic information has been carried out in biopsy specimens of benign and malignant tumors other than KS, and probable sites of HHV-8 latency in human body have been checked. HHV-8 sequences were detected by polymerase chain reaction (PCR).

Molecular characterization of Kaposi's sarcoma-associated herpesvirus/human herpesvirus-8 strains from Russia.

We report the molecular characterization, with subtyping of both K1 and K14.1/K15 genomic regions, of seven new human herpesvirus-8 (HHV-8) strains from Russian patients with classical Kaposi's sarcoma. Phylogenetic studies, based on the complete K1 gene/protein analysis, indicate that six of these strains belong to the A subtype, with one belonging to the A4 group and exhibiting a unique deletion of 19 amino acids in the VR2 region at position 186-204.

[Kinetics of the structure of neurites in mollusk brain after its dissociation].

Using phase microscopy the behaviour of living neurons directly after their enzymatic isolation from ganglia of Clione limacina and Lymnaea stagnalis was studied. Apart from single-processed unipolar neurons, numerous dipolar and unipolar ones were found in mollusc which does not conform to neuron classification generally adopted. Disrupted neurons were seen to shorten gradually in Ringer solution and -1640 medium at the rate of 0.

[Detection of markers of the human T-cell leukemia virus in patients with T-cell lymphoma and Kaposi's sarcoma].

Nine patients with Kaposi's sarcoma and five suffering from T-cell lymphoma have been examined. Antibodies to HTLV-1 were not found in these patients. The primary cellular cultures were isolated from blood and lymph nodes of the patients.

[The comparative characteristics of the clinico-immunological indices in different contingents of opisthorchiasis patients in a hyperendemic focus. 4. The transplacental transmission of Opisthorchis felineus antigens].

25 women in labour with chronic opisthorchiasis have been examined. They were representatives of native (7), local (8) and migrated population of the focus. Control group comprised 10 women from the local population free of invasion.

[The identification of calmodulin in the cytoskeletal fraction of the olfactory mucosa of vertebrates].

Calmodulin was detected in the cytoskeleton fraction of the olfactory tissue of vertebrates (pig, rat), identified by electrophoretical mobility and by immunoblotting. The approximate content of calmodulin in the cytoskeleton fraction was about 23% relative to all the proteins revealed on the electrophoregram. Another protein was detected in the same experiments having the same molecular weight as calmodulin and revealing immunospecific properties.

[A modification of an immunoenzyme test system for the serodiagnosis of opisthorchiasis].

Based on the antigen derived from the whole Opisthorchis marita extraction in cats and A-peroxidase protein conjugate the authors modified the enzyme immunoassay system for the detection of IgG antibodies to the causative agent of opisthorchiasis. A vibroshaker used in all stages of study permitted them to reduce the time of the reaction and its analysis providing the visualized consideration of the results.

[The therapeutic possibilities in lichen ruber pilaris].

The results of therapy of 43 patients with lichen ruber pilaris are analyzed. Different treatment modalities were employed: PUVA therapy, Re-PUVA therapy, retinoids, PUVA therapy with methotrexate, etc. Re-PUVA therapy was found the most effective method of treatment.

[Further cases of Kimura's disease in the USSR].

The authors review literature on the clinical picture and possible pathogenesis of Kimura's disease, as well as on therapy of the condition. They describe a case of this disease. A woman aged 37 has developed this condition with typical clinical and histological signs.

[Acne necrotica].

A 50-year-old female patient with typical symptoms of acne necrotica is described. The eruptions involved seborrheic areas on the skin of the forehead, conchae of the auricles, nose and nasolabial folds, and presented as nodules covered with serous crusts, necrotic foci, and small stamp cicatrices. Histologic findings confirm the clinical diagnosis.

[Tuberculous lupus].

A 39-year-old patient, veterinary feldsher, suffering for 3 years from tuberculosis luposa with multiple foci of involvement, is described. The process was complicated by lymphostasis and eczema on the left arm.

[Ca2+-protease--an enzyme of the metabolism of cytoskeletal proteins in the olfactory membrane of vertebrates].

Two forms of Ca(++)-activated protease (calpain I and calpain II) associated with an endogenous inhibitor (calpastatin) were detected in a cytosolic fraction of the olfactory tissue of vertebrates (pig, rat). Using ion exchange chromatography on DEAE-cellulose column, calpain I is divided into 2 peaks (eluting by 0.07-0.

[Clinico-immunologic indices of various patient contingents in a focus of chronic opisthorchiasis. 3. Dynamics of the indices following praziquantel treatment].

Studies of the time course of the immune status characteristics after praziquantel treatment and of the nature of its side-effects on various groups of opisthorchiasis patients in the focus established significant differences in their immune response to specific treatment. The lack of alterations in the T- and B-immunity systems in aboriginal population of the focus may suggest its tolerance to helminthic antigens. The trend towards cellular immunity stimulation and a significant increase in the levels of circulating immune complexes and specific antibodies in the serum were observed in aboriginal population of the focus.

[Biological characteristics of Streptococcus pneumoniae strains isolated from children with pneumococcal diseases].

The biological properties of 97 S. pneumoniae strains isolated from 92 children with purulent meningitides, meningoencephalitides, pneumonia and otitis, hospitalized at the Leningrad Research Institute of Childhood Infections, were studied. The data obtained in this investigation were indicative of the formation of atypical forms of pneumococci (R-forms, unbalanced growth forms and L-forms) in all clinical forms of pneumococcal infection in children.

[Electron microscopic study of Darier's follicular dyskeratosis].

Ultrastructure of the skin biopsy specimens of 10 patients with Darier's keratosis follicularis (DKF), 6 of them with the vesicular form of the dermatosis, has been examined by electron microscopy. The study has revealed dyskeratosis with tonofilament homogenization and well-developed keratohyalin granules, round bodies and granules, and acantholysis less manifest than in Hailey-Hailey's chronic benign familial pemphigus. Ultrastructural features of the skin in vesicular DKF present as acantholysis and dyskeratosis more manifest than in typical DKF.

[The successful treatment of cutaneous leishmaniasis with an argon laser].

A patient suffering from skin leishmaniasis is described. Clinical diagnosis was confirmed by histologic findings and isolation of Leishmania tropica. Since antibiotic therapy was ineffective, the patient was exposed to argon laser for the coagulation of nodular elements.

[Mibelli's porokeratosis].

A 44-year-old patient suffering from porokeratosis of Mibelli for 27 years is described. The eruptions have involved the skin of the buttocks and the upper and lower limb flexor surface. The clinical picture of the disease may be regarded as the classical plaque form.