Safety, Immunogenicity and Protective Activity of a Modified Trivalent Live Attenuated Influenza Vaccine for Combined Protection Against Seasonal Influenza and COVID-19 in Golden Syrian Hamsters.

Background/objectives: Influenza viruses and SARS-CoV-2 are currently cocirculating with similar seasonality, and both pathogens are characterized by a high mutational rate which results in reduced vaccine effectiveness and thus requires regular updating of vaccine compositions. Vaccine formulations combining seasonal influenza and SARS-CoV-2 strains can be considered promising and cost-effective tools for protection against both infections.

Methods: We used a licensed seasonal trivalent live attenuated influenza vaccine (3×LAIV) as a basis for the development of a modified 3×LAIV/CoV-2 vaccine, where H1N1 and H3N2 LAIV strains encoded an immunogenic cassette enriched with conserved T-cell epitopes of SARS-CoV-2, whereas a B/Victoria lineage LAIV strain was unmodified.

Safety and Immunogenicity Study of a Bivalent Vaccine for Combined Prophylaxis of COVID-19 and Influenza in Non-Human Primates.

Background: Influenza and SARS-CoV-2 viruses are two highly variable pathogens. We have developed a candidate bivalent live vaccine based on the strain of licensed A/Leningrad/17-based cold-adapted live attenuated influenza vaccine (LAIV) of H3N2 subtype, which expressed SARS-CoV-2 immunogenic T-cell epitopes. A cassette encoding fragments of S and N proteins of SARS-CoV-2 was inserted into the influenza NA gene using the P2A autocleavage site.

[Cryoglobulinemia in a case of multiple myeloma associated with viral hepatitis B].

Cryoglobulinemia is a biochemical disorder accompanying many infectious, systemic, and lymphoproliferative conditions. Cryoglobulins are proteins capable of reversible precipitation or gel formation at temperatures below 37 degrees C. There are 3 types of cryoglobulins depending on the type of immunoglobulins in their composition; hence, three types of cryoglobulinemia occurring in different diseases.

[A case report of successful surgical management for a combined wound of the neck with a rare variant of an injury of the vertebral artery].

Presented herein is a clinical case report regarding successful surgical management of a male patient presenting with a concomitant injury of the neck and involvement of the second portion of the contralateral vertebral artery.

[Mapping of the quantitative trait loci (QTL) associated with grain quality characteristics of the common wheat grown under different environmental conditions].

The quantitative trait loci (QTL) associated with individual characteristics of grain and flour quality in wheat lines grown under contrasting environmental conditions were mapped. Overall, 22 QTL that manifested under contrasting environmental conditions with various significances were detected on 10 chromosomes. Grain hardness and vitreousness were associated with three loci on chromosomes 5D, 6A, and 3A, while the gluten content, with two loci on chromosomes 5B and 7A.

[An enterosorbent with trimethylaminoethanol chloride. 2. Hypolipidemic action and effect on cholesterol absorption in the intestine (experimental data)].

Enterosorbent containing trimethyl aminoethanol chloride administered per os into rabbits accelerated the rate of experimental hypercholesterolemia regression. Moreover, this substance decreased 4-14C-cholesterol absorption in the rat intestine.

[Evaluation of various experimental dyslipoproteinemia models in the study of hypolipidemic agents].

Models of experimental dyslipoproteinemias have been developed by using non-inbred rats (males and females) and guinea-pigs. Administration of cholesterol (C) with fats and supplementation of 4-hydroxy-6-methyl-2-thiouracil to male rats and that of C in the mixture of fats to guinea-pigs have been demonstrated to lead to the development of atherogenic dyslipoproteinemias. Genfibrozil, cholestyramine and vasosan II, a new pectin-enriched formulation, promoted normalization of the impaired serum lipoprotein spectrum caused by exogenous C administration.

[Enterosorbent with immobilized trimethylaminoethanol chloride. I. Hypolipidemic properties].

Hypolipidemic properties of the synthesized enterosorbent, water soluble polymer containing covalently immobilized trimethylamine ethanol chloride, were studied. Content of cholesterol in blood was decreased by 29% and accumulation of cholesterol in liver tissue--by 22% after oral administration of the enterosorbent at a dose of 250 mg/kg into rats with hypercholesterolemia. Cholestyramine exhibited the similar effect only at a dose of 2.

[The hypolipidemic action of water-soluble enterosorbents of cholesterol and bile acids in an experiment].

Experiments on rats, guinea pigs and rabbits with experimental dyslipoproteinemia have revealed that the new water-soluble high-molecular weight polymers containing digitonin (ED) or a quaternary nitrogen atom (EA) have a more marked hypolipidemic action than cholestyramine and normalize lipoprotein metabolism. The digitonin-containing enterosorbent has been shown to decrease the absorption of 4-14C-cholesterol, while the nitrogen-containing enterosorbent diminishes the absorption of 3H-cholic acid.

[Experimental and clinical study of Vasosan P, a new form of pectin-enriched cholestyramine].

The bile acid sequestering agent Vasosan P is a new form of pectin-enriched cholestyramine (20%), saccharose (5%) and sorbic acid (0.18%). In comparison with cholestyramine Vasosan P has a more marked hypolipidemic effect in experiment.

[The experimental effect of amino acids on lipid metabolic indices].

The action of amino acids prevalent in soy bean proteins, as compared to casein, on lipid metabolism was studied. Administration of aspartate to experimental hyperlipidemic rats produced a pronounced hypocholesterolemic effect and led to an increased alpha-cholesterol level. Addition of glycine, arginine and cysteine to aspartate raised the hypocholesterolemic effect.

[The hypolipidemic and antiatherosclerotic action of absorbable and nonabsorbable substances in the intestines].

Hypolipidemic effect of the polyphenol compound triophen possessing antihypoxic activity was demonstrated in chronic experiments on rabbits. It was also shown that a new derivative of methylxanthine, which does not penetrate the blood--brain barrier, produces a hypolipidemic and antiatherosclerotic effect. Experiments on rats, guinea pigs, and rabbits with induced hyperlipidemia revealed a hypolipidemic effect of new complex compounds which are not absorbed in the intestine--steroid glycosides digitonin and tomatonin immobilized on a polymer.

[Hypolipidemic effect of the new diprophylline derivative, [2-hydroxy-3-(theophylline-7-ol] propyl ester of 4-benzylhydroxybenzoic acid].

A new derivative of diprophylline--[2-hydroxy-3(theophylline-7-ol)] propyl ester 4-benzyloxybenzoic acid was shown to decrease the content of cholesterol and of atherogenic lipoproteins in blood serum of hyperlipidemic guinea pigs. In blood serum of rabbits the new substance studied decreased cholesterol, free fatty acids and malonic dialdehyde concentrations.

[Effect of the synthetic tetradecapeptide corresponding to the amino acid sequence of human somatotropin 31-44 on rat blood and liver lipids].

Although synthetic tetradecapeptide, involving the 31-44 amino acid sequence of human growth hormone, exhibited a distinct lipolytic effect, content of triglycerides was not increased in rat blood and liver tissue during chronic experiments. At the same time, the level of atherogenic lipoproteins as well as activities of postheparin lipase and liver triglyceride lipase were also unaltered. The data obtained suggest that the tetradecapeptide studied stimulated simultaneously both lipolysis and oxidation of free fatty acids.

[Specific binding of cholesterol by enterosorbents].

Soluble polymers, modified by steroid glycosides digitonine or tomatonine (the "enterosorbents") bound cholesterol dissolved in alcohol. Rates of the binding depend on amount of the glycosides introduced into the sorbent. The digitonine or tomatonine enterosorbents decreased the cholesterol content in blood serum of hypercholesterolemic rats after per oral administration.

[Effect of arginine on the lipid and lipoprotein content of animal blood].

Arginine decreased cholesterol and triglyceride content in blood sera of intact rats and inhibited the development of hyperlipidemia provoked by Triton WR-1339 injection. In rabbits pretreated with cholesterol arginine diminished the content of blood serum cholesterol and triglycerides. Introduction of arginine to intact and hyperlipidemic guinea pigs decreased the VLDL and increased the HDL level.

[Participation of lecithin-cholesterol-acyltransferase in the redistribution of high density lipoprotein subfractions in the blood of newborn infants].

Evidence was obtained in vitro for the participation of lecithin-cholesterol acyl transferase in redistribution of subfractions of high density lipoproteins (HDLP) in blood plasma of newborn children. Content of some HDLP subfractions (HDLP 2c and HDLP 2b) was decreased and content of the other subfractions (HDLP 2a) was increased after incubation of blood plasma of the newborn children with lecithin-cholesterol acyl transferase preparation purified 100-fold.

[HDL2c--a new subclass of high density lipoproteins in the plasma of newborns].

A new subclass of high density lipoproteins designated as HLD2c was found in the human blood plasma of newborn by analytic ultracentrifugation in the flotation rate interval Fo1,20 8.0--12.0.