[Validation Study on a Method of Determination of Nosiheptide in Formula Feeds by HPLC-FL].

A determination method of nosiheptide in formula feeds by HPLC-FL was developed and validated, including an inter-laboratory study. Formula feeds were extracted with acetone after adding acetic acid. Liquid chromatographic separation was performed using a ZORBAX Eclipse XDB-C18 column, with acetonitrile and water containing acetic acid as the mobile phase.

Increased freezing tolerance through up-regulation of downstream genes via the wheat CBF gene in transgenic tobacco.

The wheat (Triticum aestivum L.) CBF gene family is assumed to play important roles in development of low-temperature and freezing tolerance through activation of the downstream Cor/Lea genes. However, no direct evidence shows association of the wheat CBF genes with stress tolerance or any interaction between wheat CBF transcription factors and Cor/Lea gene activation.

Improvement of freezing tolerance in tobacco plants expressing a cold-responsive and chloroplast-targeting protein WCOR15 of wheat.

Cold acclimation, an adaptive process for developing freezing tolerance in over-wintering plants, is associated with increased expression levels of a series of cold-responsive (Cor)/late embryogenesis abundant (Lea) genes. To investigate the function of Wcor15, a member of the wheat Cor/Lea gene family, for improvement of freezing tolerance, two types of transgenic tobacco lines expressing Wcor15-containing chimeric genes were produced and characterized. Immunoblot and gene expression analyses of a transgenic tobacco line expressing the Wcor15-GFP fusion gene under control of the CaMV35S promoter showed transport and abundant accumulation of the WCOR15 protein in the stromal compartment of the chloroplasts.