Recombinant expression using the tetrathionate hydrolase promoter in Acidithiobacillus ferrooxidans.

In the iron- and sulfur-oxidizing acidophilic chemolithoautotrophic bacterium, Acidithiobacillus ferrooxidans, tetrathionate hydrolase gene (Af-tth) is highly expressed during tetrathionate growth. The expression levels of Af-tth were specifically determined by quantitative reverse transcription-polymerase chain reaction and the expression ratios of S/Fe and SO/Fe were found to be 68 ± 21 and 181 ± 5, respectively. The transcriptional start site was identified by primer extension.