[Erythropoietin Receptor-Mediated Proliferative Signal Transduction in Leukemic Cell Line KOCL-33].

In order to investigate the expression of erythropoietin receptor(EPOR) in leukemic cell line and clarify the mechanism of proliferative signal transduction mediated by EPOR in the leukemic cell line KOCL-33, biotinylated EPO and flow cytometry were used for detection of EPOR expression, (3)H-TdR incoporation for cell proliferation, immunoprecipitation and Western blot analysis were used to investigate the tyrosine phosphorylation of signal transduction proteins as the causation of enhanced proliferation of KOCL-33 cells. There was EPOR expression and the cell proliferation could be stimulated by EPO in KOCL-33 cells. Dimerization and tyrosine phosphorylation of EPOR were found after 1 minute incubated with EPO.