Proteome profiling of bovine follicular fluid-specific proteins and their effect on in vitro embryo development.

The objective of this study was to determine the effect of bovine follicular fluid proteins (bFF) and their differently charged groups as maturation media supplements for in vitro embryo development. bFF was obtained by aspiration from large healthy follicles (4-10 mm in diameter) and was precipitated by 30-50% (NH)SO. The precipitated protein was fractionated into basic and acidic fractions by ion-exchanger columns.