[Uncertainty Evaluation and Internal Quality Control of ELISA Test for Food Allergens (in Egg and Milk) Using Control Samples].

We evaluated measurement uncertainty and performed internal quality control of an ELISA test for allergens in egg and milk, using control samples. For the evaluation of measurement uncertainty, the following three important factors were identified: 1) Differences in test-kit lots, 2) Different-day reproducibility, 3) Same-time reproducibility. A three-stage nested design was used, and the combined standard uncertainty of the three factors mentioned above was calculated based on the results obtained.

Genomic structure of the chicken slow skeletal muscle troponin T gene.

Troponin T (TnT) is a key protein for Ca(2+)-sensitive molecular switching of muscle contraction. In vertebrates, three TnT genes have been identified, which produce isoforms characteristic of cardiac, fast skeletal, and slow skeletal muscles through alternative splicing in a tissue-specific and developmentally regulated manner. The diversification of myofibers into forms with specific metabolic and contractile characteristics is thought to be closely associated with the differential expression of these TnT isoforms.