Development of RAPD-PCR assay for identifying Holstein, Angus, and Taiwan Yellow Cattle for meat adulteration detection.

Incidents of food fraud have occurred worldwide, particularly in the form of meat adulteration. In this study, molecular probes were developed using the Random amplification of polymorphic DNA (RAPD) polymerase chain reaction (PCR) technique in order to identify three beef subspecies-Holstein, Angus, and Taiwan Yellow Cattle. Four RAPD-PCR 10-nucleotide primers were chosen out of a total of 60 primers.

4α-Methylergosta-22(E),24(28)-dien-3β-ol, a New Marine Sterol from the Octocoral Nephthea columnaris.

A new marine sterol, 4a-methylergosta-22(E),24(28)-dien-3 P-ol (1), was isolated from the octocoral Nephthea columnaris. The structure of I was elucidated on the basis of spectroscopic and mass spectrometric methods.