Formation of lambda-DNA's in parallel- and crossed-line arrays by molecular combing and scanning-probe lithography.

With the combination of a molecular combing technique and scanning-probe lithographic patterning, lambda-DNA's were stretched and aligned to form line array structures on patterned organic monolayer surfaces. The pattern was generated by anodizing a silicon surface using scanning-probe lithography to implant a polar organic layer in the middle of a nonpolar layer. The molecule in the polar layer, (aminopropyl)triethoxysilane (APS), has a -NH(3)(+) terminal group, which interacts strongly with phosphate backbone of DNA and provides a site for selective attachment of DNA.