Publications by authors named "Chika Murai"
Article Synopsis
- Hypoxia is a key factor in cardiovascular diseases and tumors, triggering various cellular processes like blood formation, new blood vessel growth, and cell death.
- Hypoxia-inducible factor-1 (HIF-1) activates specific genes by binding to hypoxia response elements (HREs), which are crucial for developing gene therapies targeting hypoxia.
- Research shows that gene activation linked to HREs varies depending on the cell type and oxygen levels, leading to an in vitro method for studying the effects of different HRE copy numbers on gene expression.
The inhibitory effects of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and linoleic acid (LA) on the contractions induced by five prostanoids and U46619 (a TP receptor agonist) were examined in guinea pig gastric fundus smooth muscle (GFSM). Tension changes were isometrically measured, and the mRNA expression of prostanoid receptors was measured by RT-qPCR. DHA and EPA significantly inhibited contractions induced by the prostanoids and U46619, whereas LA inhibited those induced by prostaglandin D and U46619.
View Article and Find Full Text PDFBackground: Zinc is mainly absorbed in the duodenum and proximal jejunum, which are removed during pancreaticoduodenectomy (PD). Little is known about the adverse oral events and skin disorders caused by zinc deficiency after PD. Herein, we reviewed studies on the development of zinc deficiency after PD and reported about a patient with zinc deficiency after PD who required home intravenous zinc replacement.
View Article and Find Full Text PDFPurpose: The purpose of this pilot study was to determine the effectiveness and costs of the occupation-based practice for community dwelling frail elderly.
Design: Pilot pre-post design without a control group.
Setting: A care management center involving 37 local elderly.
Objective: HIF-1/HRE pathway is a promising target for the imaging and the treatment of intractable malignancy (HIF-1; hypoxia-inducible factor 1, HRE; hypoxia-responsive element). The purposes of our study are: (1) to assess the gene activation levels resulting from various numbers of HREs under various hypoxic conditions, (2) to evaluate the bidirectional activity of multiple HREs, and (3) to confirm whether multiple HREs can induce gene expression in vivo.
Methods: Human colon carcinoma HCT116 cells were transiently transfected by the constructs containing a firefly luciferase reporter gene and various numbers (2, 4, 6, 8, 10, and 12) of HREs (nHRE+, nHRE-).
Squamous cell carcinoma (SCC) with rhabdoid features (SCCRF) is extremely rare in the oral cavity. We report herein a case of oral SCCRF. The patient was a 69-year-old Japanese woman who had been suffering from a mass in the right lower gingiva.
View Article and Find Full Text PDFBackground: Salivary duct carcinoma with rhabdoid features is extremely rare.
Methods: We report 2 cases of salivary duct carcinoma with rhabdoid features treated at our institution.
Results: Case 1 was a 44-year-old Japanese man who had swelling in the left parotid region.
Background: We aimed to develop a mouse spontaneous liver metastasis model from an orthotopically implanted human colon cancer cell line stably expressing a human sodium/iodide symporter (NIS) reporter gene, which can be imaged with single-photon emission computed tomography (SPECT) using 99mTcO4-.
Methods: A recombinant plasmid containing a constitutively driven NIS gene (pcDNA3-NIS) was transfected into the human colon cancer cell line HCT116, and stable cell lines were established. The stable cells were subcutaneously injected into the nude mice.