Hemolytic disease of the fetus and newborn caused by anti-s antibody in a GP.Mur/Mur Thai mother and review of the prevalence of s in Thai blood donors.

Background: Low-prevalence antigen s (MNS23) is encoded by GYPB c.173C > G. Hemolytic disease of the fetus and newborn (HDFN) due to anti-s is rare.

HLA alloimmunization in patients receiving multitransfusions of red blood cells.

HLA antibodies were studied in 88 patients with chronic hemolytic anemia who received multitransfusions of red blood cells prepared by conventional (PRC-C), inverted centrifugation (LR-I) and leukocyte filter (LR-F) techniques. Their mean age was 8 years and 4 months with a duration of transfusion of 8 years. The patients were divided into five groups: group 1, receiving PRC-C (n=20); group 2, receiving LR-I (n=33); group 3, receiving LR-F (n=11); group 4, subsequently receiving LR-I and LR-F (n=10); and group 5, receiving PRC-C followed by LR-I and LR-F (n=14).

Preliminary study of HLA-ABCDR antigens in CML, ANLL, thalassemia and severe aplastic anemia in Thais.

The objective of this study was to analyse human leukocyte antigen (HLA) and disease association in common blood diseases [chronic myelogenous leukemia (CML), acute nonlymphocytic leukemia (ANLL), thalassemia and severe aplastic anemia] in Thais. The subjects were patients from the Hematological Clinic, Departments of Medicine and Pediatrics, Ramathibodi Hospital who were referred for HLA typing for bone marrow transplantation (BMT) at the Histocompatibility Laboratory from March 1988 to September 1997. A total of 129 patients had complete HLA-ABC typing.

Lewis antigens on newborn red cells.

This study aimed to screen for Lewis antigens in Thai newborns. Although, these antigens are known to be weak or absent on the red cells of newborns, we encountered a case of a Le(a+) newborn baby when testing with monoclonal antibody and human anti-Le(a). Such a finding led us to conduct this study to explore further evidence of Lewis antigens in Thai newborn red cells.

The organization of Kidney Transplantation Services at Ramathibodi Hospital: fourteen years experience on waiting list, kidney donors and kidney transplantation.

The Kidney Transplantation Program at Ramathibodi Hospital was established in 1985. By the end of 1998, there were 1,614 patients on the cumulative waiting list. The first kidney transplantation (KT) was started in 1986 by using kidney from living-related donor (LD) while cadaveric KT (CD-KT) was started in 1987.

Low cost locally prepared fibrin glue for clinical applications: reported of 145 cases. Committee of Bangkok International Hemophilia Training Center.

Fibrin glue (FG) is one of the blood products known to be very useful for local hemostatic measure and as a medically valuable tool for adhesion, sealing, anastomosis, repair microvascular and nerve grafts in medical and surgical procedures. Before 1996, FG was used to a limited extent in Thailand due to the high cost. Technology for locally prepared FG was transferred to Bangkok International Hemophilia Training Center of the World Federation of Hemophilia (IHTC-WFH) in July 1996 by Prof.

Detection of HBV genome by gene amplification method in HBsAg negative blood donors.

The incidence of post-transfusion hepatitis has been reduced greatly by screening blood donors for hepatitis B surface antigen (HBsAg). However, hepatitis B virus infection still accounts for a certain number of cases of post-transfusion hepatitis. The purpose of this study was to detect HBV DNA in the HBsAg negative blood samples by using nested PCR with two primer pairs specific to core region.

Characterization of a novel HLA-A2 variant, A*02012, in the Southern Thai-Muslim population.

Southern Thai Muslims (STM)--from Nakon Si Thammarat, whose ancestors come mainly from Malaysia--constitute more than half of all Thai Muslims which, in total, represent approximately 10% of the country's population. The most common A2 subtypes in STM were A*0203 (n=15), A*0201 (n=8) and A*0207 (n=7). In this study, samples with unexpected amplification patterns were sequenced.

Eluding transfusion-transmitted HIV infection: report of two cases.

Two hemophilia A boys (FVIII: C < 1% and 2.2%), whose ages were 12 and 14 years. old, received fresh frozen plasma of 140 ml and 210 ml, respectively, in 1989.

Crossmatching technique facilitating kidney transplantation.

Accelerated acute cellular rejection (AR) continues to be a serious problem in kidney transplantation (KT), suggesting that undetected presensitization may be encountered. The purpose of this study was to determine the most sensitive crossmatching (XM) technique to detect the preformed antibody (Ab) which may cause AR. One hundred and twenty two sera from 98 patients, on the waiting list for KT at Ramathibodi Hospital were XMed with 23 cadaveric splenic lymphocytes including 2 living related KT (LR-KT).

Panel reactive antibodies in post-kidney transplantation.

This study was aimed to evaluate the clinical relevance of the panel reactive antibodies (PRA) post kidney transplantation (KT). A total number of 90 KT recipients consisted of 71 male and 19 female patients. Thirty-two haploidentical and 3 HLA-identical pairs for living related KT and 55 cadaveric KT with 3-6 mismatched antigens were included in this study.

Increasing the chance to accept HLA-ABDR mismatched donor in kidney transplantation.

Two hundred and fifty-three kidney transplantations (KT) which included 68 (26.9%) living-related (L) and 185 (73.1%) cadaveric (C) KT with 0-6 HLA-ABDR mismatches (MM) were studied for the association of HLA-ABDR-MM specificities and the occurrence of graft rejection (GR).

HLA in southern Thai-Muslims.

Unlabelled: One hundred and two Southern Thai-Muslims (STM) from Nakhon Si Thammarat province were studied for HLA class I and II by SSP ARMS-PCR and PCR-SSO, respectively. The allele frequencies, haplotype frequencies, delta value and linkage disequilibrium between alleles were expressed. The most frequent alleles for HLA-A, HLA-B and HLA-C were A*24(02,03), A*11 (01,02), A*02(01,03,05-07,11): B*15(01,04-07,12,19,20), B*07(02-05), B*51(01-05)/B*52 (011,012); and Cw*07(01-03), Cw*04(01,02), Cw*08(01-03), respectively.

A*02 in southern Thai Muslims and central Thais.

Unlabelled: The HLA-A*02 subtyping in Thais was conducted and included in the 12th International Histocompatibility Workshop (12WS). A total of 81 randomized individuals previously serologically or DNA typed as A2 were studied for A2 subtypings. The subjects consisted of 32 Southern Thai-Muslims (STM) and 49 Central Thais (CT).

Effectiveness of leukocyte removal by Imugard III.

Unlabelled: Study was conducted to evaluate the effectiveness of Imugard III-RC (4B) to remove leukocyte from packed red cells (PRCs). The leukafiltration set, Imugard III (4B), bedside use for PRCs or whole blood was converted to laboratory use by connecting the distal end of filter tubing to the transfer bag and the proximal end of tubing to the primary blood pack by sterile connecting device to ensure that the closed system is intact. Twenty units of PRCs were prepared from CPD whole blood by removal of platelet rich plasma.

Transfusion-transmitted AIDS with blood negative for anti-HIV and HIV-antigen.

A four-year-old boy who was diagnosed with Glanzmann's thrombasthenia received supportive treatment and desmopressin for his bleeding episodes. He seldom received blood components, except for platelet concentrates, due to severe bleeding. He was exposed to 18 routine donors and 2 plateletpheresis donors who were negative for human immunodeficiency virus (HIV) antigen and anti-HIV upon screening.

HLA-B27 subtypes in Asian patients with ankylosing spondylitis. Evidence for new associations.

The aim of this study was to investigate the contribution of the different B27 subtypes to ankylosing spondylitis (AS) susceptibility. The polymerase chain reaction (PCR) in combination with the sequence-specific oligonucleotide probes (SSOs) was used to analyse the polymorphism in exon 2 and 3 of HLA-B27 in two Asian groups with different genetic HLA structures: Indian (I) and Thai (T) populations. The same number of AS patients (45) and healthy B27 positive donors (n = 17) from both populations were analysed in order to ascertain the B27 subtypes.