The immediate effects of weaning stress on the hypothalamus-pituitary-adrenal alteration of newly weaned piglets.

This study was conducted to examine the effects of weaning stress on gene expression of specific markers in hypothalamus-pituitary-adrenal (HPA) axis and neuronal activity in the newly weaned piglets. Twelve 28-days-old, newly weaned crossbred (Landrace × Yorkshire × Duroc) male piglets from 6 l (2 piglets/l) were randomly categorized into two groups: (a) weaning stress: piglets were separated from their dams, relocated and mixed with the unacquainted domestic piglets for 2 hr (stress, n = 6); (b) no-stress: piglets stayed with their dams in the farrowing house (NS; n = 6). After weaning stress, all piglets were electrically euthanized and the blood samples/HPA tissues were collected for subsequent analysis, including plasma cortisol and mRNA expression of c-fos, c-jun, corticotropin-releasing hormone (CRH), CRH receptor 1 (CRHR-1) and adrenocorticotropin hormone receptor (MC2R).