Publications by authors named "Chiara V Ragni"
Although in flies the atypical cadherin Fat is an upstream regulator of Hippo signalling, the closest mammalian homologue, Fat4, has been shown to regulate tissue polarity rather than growth. Here we show in the mouse heart that Fat4 modulates Hippo signalling to restrict growth. Fat4 mutant myocardium is thicker, with increased cardiomyocyte size and proliferation, and this is mediated by an upregulation of the transcriptional activity of Yap1, an effector of the Hippo pathway.
View Article and Find Full Text PDFArticle Synopsis
- Research in developmental biology needs effective tools to analyze images of developing tissues, as current methods struggle due to complexities in image acquisition.
- The paper introduces an automated framework using partial differential equations to accurately segment 3D microscopy images of densely packed tissues.
- This method has been successfully applied to study mouse heart development, yielding valuable quantitative and qualitative insights into cellular processes and tissue organization.
Anisotropies that underlie organ morphogenesis have been quantified in 2D, taking advantage of a reference axis. However, morphogenesis is a 3D process and it remains a challenge to analyze cell polarities in 3D. Here, we have designed a novel procedure that integrates multidisciplinary tools, including image segmentation, statistical analyses, axial clustering and correlation analysis.
View Article and Find Full Text PDF