Publications by authors named "Chiara Chemello"
Article Synopsis
- Fentanyl triggers neuroinflammation and immune activation through the TLR4/MD-2 complex, leading to side effects like tolerance and increased pain sensitivity.
- The study assessed fentanyl's effects on proinflammatory responses in rat microglia and human macrophages using techniques like real-time PCR and ELISA, while examining TLR4/MD-2 signaling pathways.
- Results showed fentanyl increases proinflammatory mediator levels and NF-κB activation linked to TLR4/MD-2, suggesting that targeting this pathway could enhance the safety and effectiveness of opioid pain management.
Aim: 2-Pentadecyl-2-oxazoline (PEA-OXA), the oxazoline derivative of N-palmitoylethanolamine, exerts anti-inflammatory activity; however, very little is known about the molecular mechanisms underlying this effect. Here, we tested the anti-neuroinflammatory effect of PEA-OXA in primary microglia and we also investigated the possible interaction of the molecule with the Toll-like receptor 4 (TLR4)-myeloid differentiation protein-2 (MD-2) complex.
Main Methods: The anti-inflammatory effect of PEA-OXA was analyzed by measuring the expression and release of pro-inflammatory mediators in primary microglia by real-time PCR and ELISA, respectively.