Publications by authors named "Chiara Carla Rospo"
An antibody specific to small-molecule inhibitor-bound TNF has enabled the development of target occupancy biomarker assays to support the development of novel treatments for autoimmune disorders. ELISAs were developed for inhibitor-bound and total TNF to determine the percentage of TNF occupancy in samples from stimulated blood. Inhibitor-saturated samples allowed measurement of total and inhibitor-bound TNF in a single electrochemiluminescence immunoassay.
View Article and Find Full Text PDFAs the number of therapeutic modalities expand, and the field of scientific research evolves toward finding treatment solutions for complex and rare disease, an ability to demonstrate efficacy through biomarker end points in clinical development studies is becoming increasingly important. Implementing flow cytometry in a clinical setting is challenging and many sponsor organizations take a hybrid approach, developing complex analytical methods internally before identifying and forming partnerships with contract research organizations to conduct the formal analytical method validation and sample bioanalysis. Ensuring that these interactions are effective is critical to the delivery of high-quality, impactful clinical data.
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- A neuroscience project investigated compounds that caused convulsions in animals, aiming to identify the underlying mechanism and evaluate premonitory signs and tolerance development.
- A series of patch clamp assays on ion channels associated with seizures and video EEG/EMG monitoring in dogs helped characterize the convulsions and confirm their central nervous system origin.
- The study found that convulsions were linked to strong inhibition of GABA channels, with EEG results revealing premonitory signs before seizures and no reduction in seizure effects over repeated dosing, leading to a decision against further development of the compounds.