Histopathological and Immunohistochemical Studies of Pineoblastoma in a Cow.

We report the first case of pineoblastoma in a cow. At necropsy, a soft, gelatinous greyish yellow, 4 × 3 × 2 cm mass was found midsagittally on the dorsal surface of the midbrain. The mass enveloped the brainstem anterior to the cerebellum, extended to the pituitary fossa and was adherent to the ventromedial aspect of the occipital lobes.

Gene silencing in Echinococcus multilocularis protoscoleces using RNA interference.

We investigated the potential of gene silencing in Echinococcus multilocularis protoscoleces using RNA interference (RNAi). For the introduction of siRNA, soaking and electroporation were first examined for their effects on the viability of protoscoleces and their efficacy for siRNA introduction. Consequently, electroporation using 100 V and 800 μF showed the optimal results.

Echinococcus multilocularis primary cells: improved isolation, small-scale cultivation and RNA interference.

In this study we demonstrate RNA interference mediated knock-down of target gene expression in Echinococcus multilocularis primary cells on both the transcriptional and translational level. In addition, we report on an improved method for generating E. multilocularis primary cell mini-aggregates from in vitro cultivated metacestode vesicles, and on the cultivation of small numbers of small interfering RNA-transfected cells in vitro over an extended period of time.

Evaluation of efficacy of bruceine A, a natural quassinoid compound extracted from a medicinal plant, Brucea javanica, for canine babesiosis.

Bruceine A, a natural quassinoid compound extracted from the dried fruits of Brucea javanica (L.) Merr., was evaluated for its antibabesial activity in vitro and in vivo.