Effects of stratified microbial extracellular polymeric substances on microalgae dominant biofilm formation and nutrients turnover under batch and semi-continuous operation.

Extracellular polymeric substances (EPS) are well-acknowledged to accelerate microalgal biofilm formation, yet specific role of stratified EPS is unknown. Bacterial biofilm stratified EPS could enrich phosphorus, whether microalgal biofilm stratified EPS could also realize phosphorus or nitrogen enrichment remains unclarified. This study investigated microalgae dominant biofilm growth characteristics and nutrients removal via inoculating microalgae and stratified bacterial EPS at various microalgae:bacteria ratios.

Protocol for extraction of gut interstitial fluid in mice with two-front nutrient supply.

The intestine features a two-front nutrient supply environment, comprising an enteral side enriched with microbial and dietary metabolites and a serosal side supplied by systemic nutrients, collectively supporting intestinal and systemic homeostasis, but there is currently no optimal approach for extracting and assessing the local intestinal microenvironment. Here, we present a protocol for constructing a nutrient supply model in mice and extracting gut interstitial fluid (GIF) via centrifugation. This model and the extracted GIF are suitable for downstream analyses.

The MADS-Box Transcription Factor EjAGL18 Negatively Regulates Malic Acid Content in Loquat by Repressing .

Malic acid is the major organic acid in loquat fruit, contributing to the sourness of fruit and affecting fruit flavor. However, the transcriptional regulation of malic acid in loquat is not well understood. Here, we discovered a MADS-box transcription factor (TF), EjAGL18, that regulated malic acid accumulation in loquat.

Automatic monitoring and on-line chiral separation of chiral drug synthesis.

Chiral synthesis of single chiral drugs offers high efficiency, controllable costs, and excellent enantioselectivity, making it crucial in the pharmaceutical industry. A significant number of studies on chiral drug synthesis primarily focuses on the design and synthesis of innovative chiral catalysts and ligands with extremely high selectivity, as well as the development of new methods and strategies. Nonetheless, the on-line monitoring of chiral drug synthesis and its underlying mechanisms remain obscure.

Algorithms for Solving the Equilibrium Composition Model of Arc Plasma.

In the present study, the Homotopy Levenberg-Marquardt Algorithm (HLMA) and the Parameter Variation Levenberg-Marquardt Algorithm (PV-LMA), both developed in the context of high-temperature composition, are proposed to address the equilibrium composition model of plasma under the condition of local thermodynamic and chemical equilibrium. This model is essentially a nonlinear system of weakly singular Jacobian matrices. The model was formulated on the basis of the Saha and Guldberg-Waage equations, integrated with Dalton's law of partial pressures, stoichiometric equilibrium, and the law of conservation of charge, resulting in a nonlinear system of equations with a weakly singular Jacobian matrix.

Heterointerface Built Up by Ultrathin Amorphous MoO Conformal Coating Enables VO·6HO with Superior Properties for Aqueous Zinc Batteries.

Layered VO·6HO is a promising candidate for aqueous zinc batteries (AZBs) but with moderate electrochemical performances. Herein, the charge storage properties of VO·6HO are markedly improved by building up the heterointerface on its surface using amorphous molybdenum trioxide as the heteromaterial. The amorphous molybdenum trioxide functioning as the proton reservoir enables the proton-involved electrochemical reactions and induces the formation of a built-in electric field along the [001] orientation at the heterointerface constructed by the (001) plane of VO·6HO, which could provide new diffusion pathways and extra sites for ion storage.

Innocuous solvent-based, low-temperature curable, and highly transparent photosensitive polyimides developed using soluble polyimides containing bio-based magnolol moieties.

Traditional photosensitive polyimide (PSPI) materials require a high curing temperature and exhibit low transparency, limiting their applications in thermally sensitive optical devices. To overcome this challenge, soluble photosensitive polyimide resins were synthesized based on the structural design of a bio-based magnolol monomer. It is noteworthy that the PI photoresist, developed by using the as-prepared polyimides and non-toxic solvents (2-acetoxy-1-methoxypropane, PGEMA) and other additives, demonstrated an impressive low-temperature curing performance (180 °C).

Less toxic combined microplastics exposure towards attached Chlorella sorokiniana in the presence of sulfamethoxazole while massive microalgal nitrous oxide emission under multiple stresses.

Microalgae-based wastewater treatment could realize simultaneous nutrients recovery and CO sequestration. However, impacts of environmental microplastics (MPs) and antibiotic co-exposure on microalgal growth, nutrients removal, intracellular nitric oxide (NO) accumulation and subsequent nitrous oxide (NO) emission are unclarified, which could greatly offset the CO sequestration benefit. To reveal the potential impacts of environmental concentrations of MPs and antibiotic co-exposure on microalgal greenhouse gas mitigation, this study investigated the effects of representative MPs (PE, PVC, PA), antibiotic sulfamethoxazole (SMX), and nitrite (NO-N) in various combinations on attached Chlorella sorokiniana growth, nutrients removal, anti-oxidative responses, and NO emission originated from intracellular NO build-up.

Genomic characterization reveals distinct mutational landscape of acral melanoma in East Asian.

Acral melanoma, the most common melanoma subtype in East Asia, is associated with a poor prognosis. This study aims to comprehensively analyze the genomic characteristics of acral melanoma in East Asians. We conduct whole-genome sequencing of 55 acral melanoma tumors and perform data mining with relevant clinical data.

The efficient separation of bioactive components from Eucommia ulmoides Oliver using membrane filtration technology and its mechanisms in preventing alcoholic liver disease.

The efficient extraction and purification of active components from Eucommia ulmoides Oliver (EUO) are crucial for their utilization. The structure and properties of the prepared EUO leaf polysaccharides (ELPs) and extractum (ELE) were comprehensively characterized in this study, and the intervention mechanism of the EUO polysaccharides and extractum in alcoholic liver disease (ALD) were investigated. The yield of EUO extractum was 24.

The Effects of Ultrasound on the Rehydration of Konjac Glucomannan/Soy Protein Isolate Gel and Simulation of Gas-Liquid Interface Evolution During the Rehydration Process.

Soy protein isolate (SPI) possesses potential gelling properties, making it suitable for gel-based applications. However, the gel network stability and mechanical properties of SPI are relatively poor and can be improved through modifications or by combining it with other polymers, such as Konjac Glucomannan (KGM). Combining SPI with KGM can overcome the poor gel network stability and mechanical properties of SPI, but it reduces the water-absorbing capacity of the gel network after drying, which affects the quality characteristics of plant-based protein rehydrated foods and limits the economic feasibility of soy protein foods.

Multi-crosslinking nanoclay/oxidized cellulose hydrogel bandage with robust mechanical strength, antibacterial and adhesive properties for emergency hemostasis.

Emergency bleeding presents significant challenges such as high blood flow and rapid hemorrhaging. However, many existing hemostatic bandages face limitations, including the uncontrolled release of hemostatic agents, insufficient mechanical strength, poor adhesion, and complex manufacturing processes. To address these limitations, we developed a multifunctional hydrogel bandage for emergency hemostasis using a one-pot synthesis method.

Accuracy analysis of the new artificial anatomical marker positioning method (shoulder-to-shoulder) in preventing leg length discrepancy in total hip arthroplasty.

Objective: By comparing the hip arthroplasty parameters planned with the AIHIP three-dimensional simulation surgery system, this study analyzes the accuracy of the new femoral-side "shoulder-to-shoulder" artificial anatomical marker positioning method in femoral-side prosthesis implantation and the prevention of leg length discrepancy in hip arthroplasty.

Methods: A retrospective collection of 47 patients who underwent initial total hip arthroplasty at our hospital from August 2020 to December 2022 and met the inclusion and exclusion criteria was used as the study subjects. The average age was 67.

NK Cell Exosomes Alleviate PD-L1 Expression and Facilitate Tumor Immunity by Repressing PI3K-AKT-mTOR Signaling.

Background: Liver cancer (LC) is a deadly malignancy with limited therapeutic options in recent years. Natural killer cell-derived exosomes (NK-exo), as an important bridge of information transmission between cells, also have a certain killing effect on tumor cells. On this basis, this study investigated the specific regulatory mechanism of NK-exo on LC cells.

Bacterial exopolysaccharides: Characteristics and antioxidant mechanism.

Bacterial exopolysaccharides (EPS) are secondary metabolites of microorganisms which play important roles in adhesion, protection, biofilm formation, and as a source of nutrition. Compared with polysaccharides obtained from animal and plant species, bacterial polysaccharides have significant advantages in terms of production cost and large-scale production due to their abundant metabolic pathways and efficient polysaccharide production capacity. Most extracellular polysaccharides are water-soluble, and some are insoluble, such as bacterial cellulose.

Transcriptomic analysis of plasma-derived small extracellular vesicles reveals the pathological characteristics of normal tension glaucoma.

Aim: Normal tension glaucoma (NTG) is a common optic neuropathy that can be challenging to diagnose due to the intraocular pressure remaining within the normal range. Early diagnosis and intervention are crucial for the effective lifelong management of patients.

Methods: This study recruited a total of 225 participants.

Solid-state fermentation of corn wet distiller grains and wheat bran with Trichoderma reesei and Candida utilis for improving feed value.

Background: Solid-state fermentation is one of the most effective methods for the high-value utilization of agro-industrial by-products. Co-fermentation of wet distiller grains and agricultural waste is an effective way to mitigate the feed shortage caused by corn consumption for bioethanol. It is still challenging to convert wet distiller grains and wheat bran to easily accessible carbon sources and adjust the balanced proportion of amino acids together by fermentation.

Role of MFN2 in bovine embryonic development and the mitigation of ER stress.

This study investigated the role of mitochondrial fusion protein-2 (MFN2) in bovine embryonic development and its relationship with endoplasmic reticulum (ER) stress, aiming to increase the efficiency of in vitro embryo culture. Western blot analysis revealed that MFN2 expression peaked at the 2-cell stage, decreased at the 4-cell stage, and gradually increased from the 6-8-cell stage to the blastocyst stage. Inhibiting MFN2 at the zygote stage reduced blastocyst formation and proliferation, and this damage was partially reversed by the ER stress protective agent TUDCA.

Prescribed Performance Control of a Human-Following Surveillance Robot with Incomplete Observation.

For people with lower limb muscle weakness, effective and timely rehabilitation intervention is essential for assisting in daily walking and facilitating recovery. Numerous studies have been conducted on rehabilitation robots; however, some critical issues in the field of human-following remain unaddressed. These include potential challenges related to the loss of sensory signals for intention recognition and the complexities associated with maintaining the relative pose of robots during the following process.

Disitamab vedotin plus toripalimab in patients with locally advanced or metastatic urothelial carcinoma (RC48-C014): a phase Ib/II dose-escalation and dose-expansion study.

Background: Human epidermal growth factor receptor 2 (HER2)-targeted antibody-drug conjugates (ADCs) such as disitamab vedotin (DV) and trastuzumab deruxtecan (T-DXd) have emerged as effective treatment options and received regulatory approvals for HER2-expressing locally advanced or metastatic urothelial carcinoma (la/mUC). In addition, ADCs in combination with immunotherapy have demonstrated antitumor activity. The current study aimed to evaluate the combination of DV and toripalimab in patients with la/mUC.

Photoproduction of Aviation Fuel β-Caryophyllene From the Eukaryotic Green Microalga Chlamydomonas reinhardtii.

β-caryophyllene is a plant-derived sesquiterpene and is regarded as a promising ingredient for aviation fuels. Microalgae can convert CO into energy-rich bioproducts through photosynthesis, making them potential platforms for the sustainable production of sesquiterpenes. However, heterologous sesquiterpene engineering in microalgae is still in its infancy, and β-caryophyllene production in eukaryotic photosynthetic microorganisms has not been reported.

An effective design strategy for thermally activated delayed fluorescence emitters with aggregation-induced emission to enable sky-blue OLEDs that achieve an EQE of nearly 30.

Pure organic thermally activated delayed fluorescence (TADF) materials hold great promise for efficient organic light-emitting diodes (OLEDs), yet developing high-performance blue TADF materials that integrate short delayed lifetime with aggregation-induced emission (AIE) properties remains a significant challenge. In this study, we developed three highly-efficient blue TADF emitters (32clCBP, 32clCXT and 32PclCXT) featuring AIE character by integrating rigid π-extended donors with different acceptors. Notably, the doped 32PclCXT film achieved an exceptionally high photoluminescence quantum efficiency of up to 99% and a short delayed lifetime of 1.