Teachers' perspectives on the implementation of the new national curriculum - Dataset from Vietnam.

This dataset provides insightful data on the reality of the new national curriculum implementation from primary and secondary school teachers' perspectives. The dataset addresses four main aspects of the new curriculum implementation from teachers' perspectives: (a) teachers' capacity in implementing the new curriculum, (b) teaching conditions in implementing the new curriculum, (c) challenges for teachers in implementing the new curriculum, and (d) teachers' desires to implement the new national curriculum successfully. The survey tools used to collect the data were developed based on a literature review of implementing a new curriculum from teachers' assessment.

Intracellular Neutralization of Ricin Toxin by Single-domain Antibodies Targeting the Active Site.

The extreme potency of the plant toxin, ricin, is due to its enzymatic subunit, RTA, which inactivates mammalian ribosomes with near-perfect efficiency. Here we characterized, at the functional and structural levels, seven alpaca single-domain antibodies (VHs) previously reported to recognize epitopes in proximity to RTA's active site. Three of the VHs, V2A11, V8E6, and V2G10, were potent inhibitors of RTA in vitro and protected Vero cells from ricin when expressed as intracellular antibodies ("intrabodies").

Structural basis for the substrate selectivity of a HAD phosphatase from Thermococcus onnurineus NA1.

Proteins in the haloalkaloic acid dehalogenase (HAD) superfamily, which is one of the largest enzyme families, is generally composed of a catalytic core domain and a cap domain. Although proteins in this family show broad substrate specificities, the mechanisms of their substrate recognition are not well understood. In this study, we identified a new substrate binding motif of HAD proteins from structural and functional analyses, and propose that this motif might be crucial for interacting with hydrophobic rings of substrates.