Surgical Phase Duration in Robot-Assisted Partial Nephrectomy: A Surgical Data Science Exploration for Clinical Relevance.

(1) Background: Surgical phases form the basic building blocks for surgical skill assessment, feedback, and teaching. The phase duration itself and its correlation with clinical parameters at diagnosis have not yet been investigated. Novel commercial platforms provide phase indications but have not been assessed for accuracy yet.

A sustainable layered nanofiber/sheet aerogels enabling repeated life cycles for effective oil/water separation.

Discarded oil-containing absorbents, which has been used in handling oil spills, are tricky to deal with and have rose global environmental concerns regarding release of microplastics. Herein, we developed a facile strategy to fabricate sustainable absorbents by a gas-inflating method, through which 2D electrospinning polycaprolactone nanofiber membranes were directly inflated into highly porous 3D nanofiber/sheet aerogels with layered long fiber structure. The membranes were inflated rapidly from a baseline porosity of 81.

Biomass Chitosan-Based Tubular/Sheet Superhydrophobic Aerogels Enable Efficient Oil/Water Separation.

Water pollution, which is caused by leakage of oily substances, has been recognized as one of the most serious global environmental pollutions endangering the ecosystem. High-quality porous materials with superwettability, which are typically constructed in the form of aerogels, hold huge potential in the field of adsorption and removal of oily substances form water. Herein, we developed a facile strategy to fabricate a novel biomass absorbent with a layered tubular/sheet structure for efficient oil/water separation.

Photothermal and Concus Finn capillary assisted superhydrophobic fibrous network enabling instant viscous oil transport for crude oil cleanup.

Rapid and effective removal of highly viscous oil spills from the sea remains a great challenge globally. Superhydrophobic materials are attractive candidates for handling oil spills, but they are restrained to recover oils with low viscosity exclusively. Herein, we report a novel polypyrrole wrapped superhydrophobic fibrous network using cross-shaped polyester fibers as starting blocks.

Biomimetic Fibrous Leaf-Vein Membrane Enabling Unidirectional Water Penetration and Effective Antibacterial PM Filtration.

Air pollution induced by pathogenic particulate matter (PM) has posed a serious threat to public health worldwide. Advanced air filters are thus required, not only exhibiting high PM capture efficiency, low breathing resistance, and high internal moisture transferring performance but also isolating and inactivating external pathogenic aerosols. In this study, we demonstrated a facile approach to construct a biomimetic fibrous leaf-vein membrane with unidirectional water penetration and effective antibacterial PM filtration by one-step electrospinning of poly(vinylidene fluoride) (PVDF)-based multilayer nanofibers.

Arabidopsis ETHYLENE INSENSITIVE 3 directly regulates the expression of PG1β-like family genes in response to aluminum stress.

The genes in the subfamily PG1β (beta subunit of poly-galacturonase isoenzyme 1) have a clear effect on the biosynthesis pathway of pectin, a main component of the cell wall. However, the detailed functions of the PG1β-like gene members in Arabidopsis (AtPG1-3) have not yet been determined. In this study, we investigated their functional roles in response to aluminum (Al) stress.

Biomass poplar catkin fiber-based superhydrophobic aerogel with tubular-lamellar interweaved neurons-like structure.

Superhydrophobic aerogels are attractive candidates in controlling oil spills. The major challenges for existing aerogels are the construction of mechanical endurance as well as accessible of building materials. Herein, a newfangled biomass superhydrophobic aerogel (M-PCF/CS) with both superior compressibility and oil caption speed is fabricated by assembling poplar catkin fiber (PCF) hollowed-out shell of 330 nm and chitosan (CS) into tubular-lamellar interweaved neurons-like structure.

The complete plastid genome and phylogenetic analysis of .

is an economically important species of macroalgae. The plastid genome sequence of is 185,640 bp with a GC content of 29.34%.

Transcriptomic and Proteomic Analysis of Mannitol-metabolism-associated Genes in Saccharina japonica.

As a carbon-storage compound and osmoprotectant in brown algae, mannitol is synthesized and then accumulated at high levels in Saccharina japonica (Sja); however, the underlying control mechanisms have not been studied. Our analysis of genomic and transcriptomic data from Sja shows that mannitol metabolism is a cyclic pathway composed of four distinct steps. A mannitol-1-phosphate dehydrogenase (M1PDH2) and two mannitol-1-phosphatases (M1Pase1 and MIPase2) work together or in combination to exhibit full enzymatic properties.

Ethylene insensitive3-like2 (OsEIL2) confers stress sensitivity by regulating OsBURP16, the β subunit of polygalacturonase (PG1β-like) subfamily gene in rice.

The transcription factors EIN3 (ETHYLENE-INSENSITIVE 3) and EILs (EIN3-Likes) play important roles in plant development and defense responses; however, their mechanism in these processes remain unclear. Here, we report that OsEIL2, an EIN3-like transcription factor from rice (Oryza sativa), plays important roles in abiotic stress and leaf senescence. OsEIL2 is a nuclear-localized protein with transactivation activity in the C-terminus (amino acids 344-583) and can be induced by NaCl, polyethylene glycol (PEG), dark, and abscisic acid (ABA) treatment.

Evolution of Complex Thallus Alga: Genome Sequencing of .

, as one of the most important brown algae (Phaeophyceae) with multicellular thallus, has a very remarkable evolutionary history, and globally accounts for most of the economic marine aquaculture production worldwide. Here, we present the 580.5 million base pairs of genome sequence of , whose current assembly contains 35,725 protein-coding genes.

Phytomelatonin receptor PMTR1-mediated signaling regulates stomatal closure in Arabidopsis thaliana.

Melatonin has been detected in plants in 1995; however, the function and signaling pathway of this putative phytohormone are largely undetermined due to a lack of knowledge about its receptor. Here, we discovered the first phytomelatonin receptor (CAND2/PMTR1) in Arabidopsis thaliana and found that melatonin governs the receptor-dependent stomatal closure. The application of melatonin induced stomatal closure through the heterotrimeric G protein α subunit-regulated H O and Ca signals.

Complete sequences of the mitochondrial DNA of the .

Here, the complete mitogenome of (J. Agardh) K. L.

Complete sequences of the mitochondrial DNA of the (Rhodophyta).

In this study, we sequenced and analyzed the complete mitogenome of (Lamouroux) C. Agardh. The complete mitogenome was 29,274-bp long, containing 51 genes, including 24 protein-coding genes, 1 intron, 2 rRNA genes, 24 tRNA genes, and 1 unidentified open reading frame.

The complete mitochondrial genome of the economic red alga, .

is an economically important marine alga. In this study, we obtained complete mitogenome of by high-throughput sequencing, which was mapped as a circular molecule of 26,897 bp with 27.56% GC content and was identified 53 genes, including 25 protein-coding genes, 2 rRNA genes, 26 tRNA genes, and 1 group II intron inserted into the gene.

Complete mitochondrial genome of cultivar 'Hainong No.1' ( × ).

Here, we sequenced the complete mitogenome of 'Hainong No.1', a hybrid cultivar produced by crossing the cultivars 'Zaohoucheng' (♂) and 'Pingbancai' (♀). Circular mapping revealed that the mitogenome was 37,657 bp in length and had an overall AT content of 64.

Functional genomics analysis reveals the biosynthesis pathways of important cellular components (alginate and fucoidan) of Saccharina.

Although alginate and fucoidan are unique cellular components and have important biological significance in brown algae, and many possible involved genes are present in brown algal genomes, their functions and regulatory mechanisms have not been fully revealed. Both polysaccharides may play important roles in the evolution of multicellular brown algae, but specific and in-depth studies are still limited. In this study, a functional genomics analysis of alginate and fucoidan biosynthesis routes was conducted in Saccharina, and the key events in these pathways in brown algae were identified.

Complete mitochondrial genome of Kjellmaniella crassifolia (Laminariaceae, Phaeophyceae): Laminaria and Saccharina are distinct genus.

The kelp Kjellmaniella crassifolia is one of the most important economic resources, which is widely distributed in Japan and has now been introduced to coastal areas in China's Shandong Province for cultivation. In this study, we present the complete mitochondrial genome of K. crassifolia.

Complete Plastid Genome of the Brown Alga Costaria costata (Laminariales, Phaeophyceae).

Costaria costata is a commercially and industrially important brown alga. In this study, we used next-generation sequencing to determine the complete plastid genome of C. costata.

Complete Plastid Genome Sequence of the Brown Alga Undaria pinnatifida.

In this study, we fully sequenced the circular plastid genome of a brown alga, Undaria pinnatifida. The genome is 130,383 base pairs (bp) in size; it contains a large single-copy (LSC, 76,598 bp) and a small single-copy region (SSC, 42,977 bp), separated by two inverted repeats (IRa and IRb: 5,404 bp). The genome contains 139 protein-coding, 28 tRNA, and 6 rRNA genes; none of these genes contains introns.

Genetic Map Construction and Quantitative Trait Locus (QTL) Detection of Six Economic Traits Using an F2 Population of the Hybrid from Saccharina longissima and Saccharina japonica.

Saccharina (Laminaria) is one of the most important economic seaweeds. Previously, four genetic linkage maps of Saccharina have been constructed and five QTLs have been identified. However, they were not enough for its breeding.

Phylogeny of C4-photosynthesis enzymes based on algal transcriptomic and genomic data supports an archaeal/proteobacterial origin and multiple duplication for most C4-related genes.

Both Calvin-Benson-Bassham (C3) and Hatch-Slack (C4) cycles are most important autotrophic CO2 fixation pathways on today's Earth. C3 cycle is believed to be originated from cyanobacterial endosymbiosis. However, studies on evolution of different biochemical variants of C4 photosynthesis are limited to tracheophytes and origins of C4-cycle genes are not clear till now.

Quaternized chitosan oligomers as novel elicitors inducing protection against B. cinerea in Arabidopsis.

Chitosan oligomers prepared from enzyme hydrolysis of chitosan have for many years been recognized as potent elicitors of plant innate immunity, but their efficacy is limited by the degree of polymerization and the degree of acetylation. In this study, we presented a new type of chitosan oligomers (COS), with the name of quaternized chitosan oligomers (QCOS) that were prepared by reacting COS with glycidyltrimethylammonium chloride and overcome these problems. First, QCOS clearly induced hydrogen peroxide accumulation and callose deposition in Arabidopsis seedlings.

OsGA2ox5, a gibberellin metabolism enzyme, is involved in plant growth, the root gravity response and salt stress.

Gibberellin (GA) 2-oxidases play an important role in the GA catabolic pathway through 2β-hydroxylation. There are two classes of GA2oxs, i.e.

Complete mitochondrial genome of Undaria pinnatifida (Alariaceae, Laminariales, Phaeophyceae).

Undaria pinnatifida is one of the most important economic marine algae and key components of coastal ecosystems. Undaria pinnatifida owns a typical heteromorphic, diplohaplontic life cycle. We present the complete sequence of mitochondrial genome of U.